SMALL BIOMOLECULE HIGH INTENSITY DIFFRACTOMETER

请求提供资金，用于购买一台单晶X射线 衍射仪与高功率旋转阳极发电机和低 温度附件。目前的设备是一个旧的衍射仪 用弱的常规X射线源 它由一个 20岁，过时的计算机，失败越来越频繁， 由于缺少零件，很难修理。 此请求 仪器将首次使埃默里大学的8名研究人员 与13个NIH支持的研究项目相关的大学， 无数原子水平的详细结构信息 结晶样品（i）太小（平均边长< 0.1 mm）（ii） 衍射太弱，或（iii）相对于 去溶剂化或热分解将由现有的 埃默里大学的X射线晶体学能力。收购 详细的X射线晶体结构信息现在已经成功 限制因素，在一些程序和要求 仪器仪表将有直接和持久的影响， 大量资助研究的有效性。 受影响的NIH项目包括：（i）开发抗艾滋病药物 和用于肾成像的诊断剂，（ii）合成 抗癌药物、DNA结构的分子探针和模型化合物 铁中心的金属蛋白质，和（iii）调查 与脂类有关的分子聚集体。 X射线设施将设在化学系， 由经过培训的内部工作人员仔细运行和维护， 这个目的。 该设施将提供给其他一些 埃默里大学姐妹机构的生物医学研究人员 （埃默里医学院，疾病控制中心，美国 癌症协会，埃默里耶基斯灵长类动物中心，和退伍军人的 管理医学中心）和其他NIH资助的研究人员， 亚特兰大地区。

Funds are requested for the purchase of a single crystal X-ray diffractometer with a high power rotating anode generator and a low temperature accessory. The present facility is an old diffractometer with a weak conventional X-ray source. It is controlled by a twenty-year old, obsolete computer, which fails increasingly often and is difficult to repair for lack of spare parts. This requested instrumentation will enable for the first time 8 investigators at Emory University associated with 13 NIH supported research programs to garner detailed structural information at an atomic level for a myriad of crystalline samples which are (i) too small (< 0.1 mm average side) (ii) too weakly diffracting, or (iii) too unstable with respect to desolvation or thermal decomposition to be examined by the existing X-ray crystallographic capabilities at Emory. The acquisition of detailed X-ray crystallographic structural information is now a success limiting factor in a number of programs and the requested instrumentation will have immediate as well as lasting impact on the efficacy of considerable funded research. Affected NIH programs include (i) the development of anti-AIDS agents and diagnostic agents for renal imaging, (ii) the synthesis of anticancer drugs, molecular probes of DNA structure, and model compounds of iron centers in metalloproteins, and (iii) the investigation of molecular aggregates related to lipids. The X-ray facility will be housed in the Chemistry Department, and carefully run and maintained by in-house staff who will be trained for this purpose. The facility will be made available to a number of other biomedical investigators at Emory University's sister institutions (Emory Medical School, the Centers for Disease Control, the American Cancer Society, the Emory Yerkes Primate Center, and the Veteran's Administration Medical Center) and to other NIH-funded investigators in the Atlanta area.