LARGE-SCALE ANIMAL CELL PRODUCTION FOR BASIC RESEARCH

美国国家科学基金会于1974年成立了细胞培养中心 并继续支持它，目的是提供大型 科学家缺乏大量动物细胞及其产品 生产自己的细胞的设施。按照从一开始就计划的，NSF 资金现在正在逐步取消。在过去的九年里，该中心已经 完成了300多个涉及大规模动物细胞的项目 制作。许多材料已经提供给了外面的研究人员 新英格兰地区，几乎所有人都得到了NIH的支持。自NSF以来 1986年后，资金将不再继续，我们正在寻求部分支持 来自国立卫生研究院的中心。我们认为，这种支持将具有成本效益。 因为大规模细胞和病毒制备可以处理得更多 该中心比个别调查人员更有效率。 在未来五年内，该中心计划达到或超过其目前的 每年生产一万亿个细胞。此外，广泛的努力 将通过开展研究来改善提供的服务。 以下几个方面：(1)减少细胞生产所需的血清； (2)微载体和其他细胞生产的发展和应用 系统；(3)使用计算机技术和仪器来优化 细胞生产的条件。

The National Science Foundation established the Cell Culture Center in 1974 and has continued to support it for the purpose of providing large quantities of animal cells and their products to scientists lacking facilities to produce their own cells. As planned from the beginning, NSF funding is now being phased out. In the past nine years, the Center has completed more than 300 projects involving large-scale animal cell production. Much of the material has been supplied to researchers outside the New England area, almost all of whom have NIH support. Since NSF funding will not continue after 1986, we are seeking partial support for the Center from NIH. We feel that this support will be cost effective since large-scale cell and virus preparation can be handled much more efficiently by the Center than by individual investigators. Over the next five years, the Center plans to meet or exceed its current annual production rate of a trillion cells. In addition, extensive efforts will be made to improve the services provided by conducting research in the following areas: (1) Reduction in serum requirements for cell production; (2) Development and application of microcarriers and other cell production systems; (3) The use of computer technology and instrumentation to optimize conditions for cell production.