DYNAMICS OF PHASE SEPARATION IN MODEL BIOLOGICAL SYSTEMS

模型生物系统中相分离的动力学

• 批准号: 3916082
• 负责人: ELEANOR D SIEBERT
• 金额: --
• 依托单位: MOUNT SAINT MARY'S UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916082
• 关键词: biological models; cholesterol; crystallization; infrared spectrometry; ionic strengths; light scattering; liquid crystal; pathologic process; physical separation; solvents; temperature; ultraviolet spectrometry

The long-term objective of this project is to elucidate certain aspects of the dynamics of phase separation. The proposed research will consider the separation of anhydrous cholesterol crystals from solutions which model, to some degree, biological systems. Three specific aims of the proposed work are to investigate the effect of: (1) molecular aggregation in the one-phase region on phase separation, (2) degree of supersaturation on initial rates of crystal growth, and (3) ionic strength of solvent on molecular aggregation and crystal growth. Solutions will be studied in the one-phase region where cholesterol forms molecular aggregates, using infrared and ultraviolet spectroscopy. The effect of temperature and ionic strength on the short-range structure of the liquid state will be studied as the solution approaches the limit of practical supersaturation. The scattering of light just above the temperature of phase separation may give some insight to fluctuations in the one-phase region responsible for nucleation of the new phase; similar studies in the two-phase region will probe the early stages of phase separation. Optical studies of crystal growth allow a measurement of crystal growth and size distribution under various conditions of interest. The insignificance of the proposed research may be viewed in terms of its contribution to the basic understanding of the dynamics of phase separation, as well as to the understanding of lipid deposition in biological systems. The project will involve studies of the basic physical process, phase separation, in what may serve as model biological systems. The results of the studies will provide potentially important information to the understanding and control of cholestrol-based diseases of the body.

这个项目的长期目标是阐明某些方面 相分离的动力学。拟议的研究将考虑 从无水胆固醇晶体的溶液中分离 在某种程度上，是生物系统的模型。的三个具体目标 建议的工作是研究：(1)分子聚集的影响 在单相区上的相分离，(2)程度 晶体生长初始速率的过饱和度，以及(3)离子 溶剂对分子聚集和晶体生长的影响。 解决方案将在形成胆固醇的单相区进行研究 分子聚集体，使用红外和紫外光谱。这个 温度和离子强度对晶体短程结构的影响 当溶液接近极限时，将研究液体状态。 实际的过饱和度。光的散射恰好在 相分离的温度可能会给出一些关于 负责新相成核的单相区；类似的 对两相区域的研究将探索阶段的早期阶段 分离。晶体生长的光学研究允许测量 不同条件下的晶体生长和尺寸分布 利息。 这项拟议研究的无关紧要之处可以从它的 对基本理解相动力学的贡献 分离，以及对脂肪沉积的理解 生物系统。该项目将涉及对基础设施的研究 物理过程，相分离，可以作为生物模型 系统。研究的结果将提供潜在的重要信息 了解和控制以胆固醇为基础的疾病的信息 在身体上。