HYPOTHALAMIC AMINERGIC AND PEPTIDERGIC ALTERATIONS

下丘脑胺能和肽能改变

• 批准号: 3960162
• 负责人: DAVID K SUNDBERG
• 金额: --
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3960162
• 关键词: aging; animal old age; beta antiadrenergic agent; catecholamines; drug interactions; high performance liquid chromatography; hypothalamic hormones; hypothalamus; juvenile animal; mature animal; neuroendocrine system; neuropharmacology; neurotransmitter metabolism; neurotransmitters; radioimmunoassay; radiotracer; steroids; stress; tyrosine; vasopressins

The applicant proposes to study the effects of age and stress on catecholamine metabolism and hypothalamic peptide concentrations. He proposes to measure (1) hypothalamic amine biosynthesis and peptide (in particular LHRH, oxytocin, somatostatin, vasopressin, thyrotropin releasing hormone and enkephalins) content in rats of different ages using an in vitro methodology which he has developed and used previously for other studies; (2) the effect of acute and chronic stress on these parameters; (3) the efficacy of opioid agonists on this system in animals of various age with and without stress; and (4) effects of pharmacological intervention on stress and aging changes.

申请人建议研究年龄和压力对 儿茶酚胺代谢和下丘脑多肽浓度。他 建议测量(1)下丘脑胺的生物合成和多肽(in 特异性LHRH、催产素、生长抑素、加压素、促甲状腺激素释放 不同年龄大鼠激素和脑啡肽)含量的体外研究 他以前开发并用于其他研究的方法； (2)急、慢性应激对上述指标的影响； 阿片类激动剂对不同年龄动物中枢神经系统的影响 且无应激；(4)药物干预的影响。 压力和衰老会发生变化。