MULTIPURPOSE CLONING/SUBCLONING SYSTEM

多功能克隆/亚克隆系统

• 批准号: 3638342
• 负责人: RODERIC K M DALE
• 金额: $ 4.64万
• 依托单位: BIOTIX
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3638342
• 关键词: bacteriophage lambda; biological products; molecular cloning

This proposal is designed to develop systems for cloning stable small and large DNA fragments, which also are optimized for producing a series of overlapping subclones, using either the single stranded technique or the double stranded procedure. Vectors will be constructed from existing vectors, using a powerful new insertion technique. Construction success will lead to development and production of integrated kits. Phase I objectives are construction of a vector with a multiple cloning region, optimized for both single and double stranded subcloning procedures, and modifying the vector to includes a cos site to permit up to 40kb fragments to be cloned stably.

本提案旨在开发克隆稳定的系统 小的和大的DNA片段，它们也被优化， 使用单个克隆产生一系列重叠的亚克隆 链技术或双链程序。 载体将 从现有的载体构建，使用强大的新的 插入技术 建设成功将导致 开发和生产集成套件。 第一阶段目标 是构建具有多克隆区的载体， 优化单链和双链亚克隆 程序，并修改该载体，以包括一个cos位点， 允许稳定克隆高达40kb的片段。