MULTIPURPOSE CLONING/SUBCLONING SYSTEM

本提案旨在开发克隆稳定的系统 小的和大的DNA片段，它们也被优化， 产生一系列重叠的亚克隆，使用单一的 链技术或双链程序。 载体将 从现有的载体构建，使用强大的新的 插入技术 建设成功将导致 开发和生产集成套件。 第一阶段目标 是构建具有多克隆区的载体， 优化单链和双链亚克隆 程序，并修改该载体，以包括一个cos位点， 允许稳定克隆高达40kb的片段。

This proposal is designed to develop systems for cloning stable small and large DNA fragments, which also are optimized for producing a series of overlapping subclones, using either the single stranded technique or the double stranded procedure. Vectors will be constructed from existing vectors, using a powerful new insertion technique. Construction success will lead to development and production of integrated kits. Phase I objectives are construction of a vector with a multiple cloning region, optimized for both single and double stranded subcloning procedures, and modifying the vector to includes a cos site to permit up to 40kb fragments to be cloned stably.