CHEMOPREVENTION OF MNU-INDUCED MAMMARY TUMORS IN THE RAT

MNU 诱导的大鼠乳腺肿瘤的化学预防

• 批准号: 2302625
• 负责人: G CLINTON
• 金额: $ 39.15万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1997-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2302625
• 关键词: CHEMOPREVENTION; MNU; INDUCED; MAMMARY; TUMORS

Chemoprevention of MNU-Induced Mammary Tumors in the Rat by combinations of chemopreventive Agents (Workstatement #61) Virgin, female Sprague- Dawley rats are being used to determine the efficacy of a series of combinations-of chemopreventive agents to prevent mammary cancer. The animals are being fed a diet consisting of standard laboratory chow (e.g. Teklad 4% Rodent Chow). The carcinogen, N-methyl-N-nitrosourea (MNU), administered at about 120 days of age, is used to induce mammary tumors . An MTD for each combination of agents is established and the chemoprevention experiment shall proceed at 0.8 MTD and 0.4 MTD of each combination. Doses for both the rangefinding and the combination tests shall be approved by the Project Officer. The chemopreventive agents is administered starting l week prior to the first carcinogen dose and given to the end of the experiment. The 9-cis-retinoic acid, DHEA, tamoxifen are being administered in the diet. Indole-3-carbinol and vorozole will be administered i.g. on a constant mg/kg body weight basis. Blood levels and food consumption are being determined for each agent (where practicable). The rats are sacrificed at the end of the experiment at 150 days after the carcinogen dose or when moribund. The number of mammary tumors per animal, and the percent of animals with tumors, and the mean tumor latency (mean time to first tumor) shall be recorded for each group. The mammary tumors shall be excised and a mean tumor weight established for each group. A histological diagnosis shall be made on each tumor.

联合用药预防MNU诱发的大鼠乳腺肿瘤 化学预防药物(工作说明#61)处女，斯普拉格- 道利大鼠正被用来确定一系列 化学预防药物的组合，以预防乳腺癌。这个 动物被喂以由标准实验室食物组成的日粮(例如 泰克拉4%啮齿动物周氏)。致癌物质N-甲基-N-亚硝脲(MNU)， 在大约120天龄时服用，用于诱发乳腺肿瘤。 为每种代理组合建立MTD，并且 化学预防试验应分别在0.8MTD和0.4MTD进行 组合。测距和组合测试的剂量 应由项目官员批准。化学防护剂是 在第一次致癌剂量前一周开始使用L，并给予 直到实验结束。9-顺式维甲酸、脱氢表雄酮、三苯氧胺 在饮食中给药。吲哚-3-甲醇和伏拉唑 灌胃给药。在恒定毫克/公斤体重的基础上。血液水平和 正在确定每种制剂的食物消耗量(在可行的情况下)。 实验结束后150天处死大鼠。 致癌剂量或垂死时。每个人的乳腺肿瘤数量 动物，以及有肿瘤的动物的百分比，以及平均肿瘤潜伏期 (平均至第一次肿瘤的时间)应为每组记录。乳房 应切除肿瘤，并确定每个肿瘤的平均重量 一群人。应对每个肿瘤进行组织学诊断。