CORE--PHARMACOLOGY LABORATORY

加州大学旧金山分校核心药理学实验室 弗朗西斯科将负责药物浓度测量和 支持药物药理研究的药代动力学分析 用于治疗艾滋病毒感染和相关的机会主义 感染。该实验室将作为ACTG的中心设施 年药物和代谢物浓度的常规分析试验 生物液体(如齐多夫定)，并将负责 开发和实施新的化学或生物检测方法 治疗剂。此外，实验室人员将提供 咨询研究设计和分析，包括执行 药代动力学和药效学分析。 核心药理实验室股一直是核心实验室设施。 在过去的5年里为ACTG工作。这个实验室的工作人员是 具有化学和生物检测开发和常规药物方面的经验 液位测量已开发或实施了多项分析 药物，如齐多夫定(ZDV)、GZDV、Foscarnet、AzdU、GM-CSF、氨苯砜、 甲氧苄氨嘧啶和磺胺甲恶唑。目前正在使用的分析方法 包括高效液相色谱、气相色谱、酶联免疫吸附试验、放射免疫分析和荧光 极化。所有新药测试开发将由 经验丰富的研究化学家，由员工研究助理执行 ZDV及其胞内病毒检测方法的研究进展 单核/巨噬细胞和淋巴细胞组分中的磷酸化合成代谢产物。 用于药代动力学分析的程序包括隔室和 使用PC NONLINR等微型计算机程序的非隔室方法 和西帕尔。用于基于人群的药代动力学研究和 将使用药效学研究、NONMEM和EMAX建模程序。

The Core Pharmacology Laboratory Unit of the University of California, San Francisco will be responsible for drug concentration measurements and pharmacokinetic analysis to support pharmacologic investigations of drugs used in the treatment of HIV infection and associated opportunistic infections. The laboratory will function as a central facility for ACTG trials for the routine analysis of drug and metabolite concentrations in biological fluids (e.g. zidovudine) and will be responsible for the development and implementation of chemical or biological assays for new therapeutic agents. In addition, laboratory personnel will provide consultation on study design and analysis including performance of pharmacokinetic and pharmacodynamic analysis. The Core Pharmacology Laboratory Unit has been a core laboratory facility for the ACTG for the last 5 years. Personnel of this laboratory are experienced in chemical and biological assay development and routine drug level measurement having developed or implemented assays for numerous agents, e.g. zidovudine (ZDV), GZDV, foscarnet, AzdU, GM-CSF, dapsone, trimethoprim, and sulfamethoxazole. Assay methodologies currently in use include HPLC, gas chromatography, ELISA, radioimmunoassay, and fluorescent polarization. All new drug assay development will be carried out by experienced research chemists and performed by staff research associates assay development for measurement of intracellular ZDV and its phosphorylated anabolites in monocyte/macrophage and lymphocyte fractions. Procedures utilized for pharmacokinetic analysis include compartmental and non-compartmental methods using microcomputer programs such as PC NONLINR and SIPHARR. For population based pharmacokinetic studies and pharmacodynamic studies, NONMEM and Emax modeling procedures will be used.