INACTIVATED PNEUMOLYSIN-POLYSACCHARIDE CONJUGATE PNEUMOCOCCAL VACCINE

肺炎溶血素(Ply)在所有致病性肺炎球菌中都已被发现。 分离株。这个53,000 mW的蛋白质是一种硫醇激活的细胞溶血素。它 属于一类细菌细胞溶血素，包括：产气荚膜溶血素， 李斯特菌溶血素和肺泡溶血素的特征是它们有能力 绵羊红细胞溶解(SRBC)。有人提议，一项 灭活的溶血素可以提供一种蛋白质载体 肺炎球菌多糖结合疫苗。单抗Ig G和Ig M 在注射9V型多糖(PS)的小鼠中产生抗体 9VPS与灭活肺溶血素偶联。它们的绑定 对第9族交叉反应PSS的活性进行了研究。免疫球蛋白和免疫球蛋白M 在小鼠腹水中产生单抗。一个 腹水1：2000稀释度显示9VPS抗体为0.45- 0.65 OD 405 nm酶联免疫吸附试验单位，37℃孵育1小时。抑制力 9V型抗原抗体交叉反应组9PSS 检查过了。9VPS预处理可吸收大部分9V抗体，并引起 对9VPS-9V单抗反应的抑制率为72.9-75.0%。在免疫学中 9VPS单抗、9APS诱导的反应抑制率为69.5%，9LPS诱导的反应抑制率为69.5% 19.2%和9NPS几乎没有抑制作用。在反应中使用了兔子 9V抗血清和9VPS对抗原抗体反应的抑制作用 9LPS和9NPS为57.8%~62.7%。这些交叉反应的PSS显示 兔抗血清对该反应有较高的抑制作用。类型 9V单抗诱导中性粒细胞活化的实验研究 吞噬细胞杀灭9V肺炎球菌的活性。

Pneumolysin (Ply) has been identified in all pathogenic pneumococcal isolates. This 53,000 MW protein is a thiol-activated cytolysin. It belongs to a class of bacterial cytolysins including: perfringolysin, listeriolysin and alveolysin which are characterized by their ability to lyse Sheep red blood cells (SRBC). It has been proposed that an inactivated pneumolysin could provide a protein carrier for a pneumococcal polysaccharide conjugate vaccine. Monoclonal IgG and IgM antibodies to type 9V polysaccharide (PS) were produced in mice injected with type 9VPS conjugated to inactivated pneumolysin. Their binding activities to group 9 cross-reactive PSs were studied. The IgG and IgM monoclonal antibodies (MAb) were produced in mouse ascites fluid. A dilution of 1:2000 of the ascites fluid showed 9VPS antibodies of 0.45- 0.65 OD 405nm ELISA unit when incubated at 37 C for 1 hr. The inhibition of type 9V antigen-antibody reaction by cross-reactive group 9 PSs was examined. Pre-treatment with 9VPS absorbed most of 9V Ab and caused 72.9-75.0% inhibition of the 9VPS-9V MAb reaction. In the immunologic reaction using 9VPS MAb, 9APS induced 69.5% inhibition, 9LPS induced 19.2% and 9NPS caused almost no inhibition. In the reaction using rabbit 9V antiserum and 9VPS, the inhibition of the antigen-antibody reaction by 9LPS and 9NPS ranged from 57.8-62.7%. These cross-reactive PSs showed much higher inhibition in the reaction using rabbit antiserum. The type 9V IgG MAb stronly induced activation of PMN leukocytes for opsonophagocytic activity to kill 9V pneumococci.