FIELD-APPLICABLE SEROLOGIC DIAGNOSIS AND EXPERIMENTAL CONTROL STRATEGIES

现场适用的血清学诊断和实验控制策略

• 批准号: 3747614
• 负责人: ROBERT H GILMAN
• 金额: --
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3747614
• 关键词: Peru; anthelmintics; clinical trials; communicable disease control; cooperative study; diagnosis design /evaluation; diagnostic tests; disease reservoirs; epidemiology; feces analysis; field study; health education; helminthiasis; human subject; human therapy evaluation; humoral immunity; immunoglobulin G; immunoglobulin isotypes; nonhuman therapy evaluation; parasitic disease chemotherapy; parasitic disease diagnosis; serology /serodiagnosis; swine; western blottings

Human Taenia solium cysticercosis is a severely debilitating, yet treatable disease. In the pig, this infection accounts for multimillion dollars of economic loss per year. Preliminary epidemiological surveys with the newly developed immmunoblot assay (EITB) in many Latin American countries, China, and India indicated that this is a significant public health problem with prevalence rates ranging from 8% to 15% in humans and up to 45% in pigs. A recent Centers for Disease Control Survey showed endogenous transmission in families with immigrant domestic workers, resulting in a sero- prevalence of 13% in a single community in Brooklyn alone. Cysticercosis has therefore, acquired a domestic mode. Control of this important public health problem is dependent on the identification and treatment of patient and pigs. We propose to extend and enhance the power, usefulness, and practicality of our unique assay/field operation system toward the detection and control of this important disease. We also propose to test the efficacy of a novel pig/human approach to surveillance and control of cysticercosis. We will: 1. To develop a low cost, robust, and field applicable version of the EITB assay for the detection of human and pig cysticercosis 2, determine the longevity of infection-specific antibodies in treated patients by quantitative isotype assays 3, To test the hypothesis that a single treatment of EITB positive pigs, together with elimination of tape worms in sero-positive human patients, and eduction is a viable and effective approach to the control of human and pig cysticercosis 4, to determine if the serology of cysticercosis-free pigs is a useful and effective sentinel for measuring disease prevalence and control effectiveness of human cysticercosis.

人类猪带绦虫囊虫病是一种严重的衰弱，但 可治疗的疾病 在猪中，这种感染占数百万 每年的经济损失。 初步流行病学调查 与新开发的免疫印迹分析（EITB）在许多拉丁美洲 中国和印度表示，这是一个重大的公共 健康问题，人类患病率为8%至15%， 在猪中高达45%。 疾病控制中心最近的一项调查显示，内源性传播 在有移民家庭佣工的家庭中， 仅布鲁克林一个社区的患病率就达到了13%。 囊虫病 因此，获得了国内模式。 控制这个重要的公众 健康问题取决于患者的识别和治疗 还有猪 我们建议扩大和加强权力，有用性和实用性 我们独特的检测/现场操作系统， 控制这一重大疾病。 我们还建议测试 一种新的猪/人方法来监测和控制 囊虫病 我们将：1.开发一个低成本、可靠和现场适用的版本 用于检测人和猪囊虫病2的EITB测定， 确定感染特异性抗体的寿命， 通过定量同种型测定3，为了检验假设， EITB阳性猪的单次给药，同时消除胶带 蠕虫在血清阳性的人类患者，和教育是一个可行的， 有效控制人和猪囊虫病的方法4， 确定无囊虫病猪的血清学是否是有用的， 衡量疾病流行和控制的有效哨点 人类囊虫病的有效性。