PURCHASE OF AN ANLYTICAL ULTRACENTRIFUGE

购买分析超速离心机

• 批准号: 2284194
• 负责人: Edward Eisenstein
• 金额: $ 13.7万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-18 至 1995-05-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2284194
• 关键词: analytical ultracentrifugation; biomedical equipment purchase; structural biology

A major users group of nine structural biologists from the three campuses in the University of Maryland System seeks funding for an analytical ultracentrifuge to replace our 40 year old Model E ultracentrifuge at CARB. The major users group consists of Drs. Armstrong and Julin from College Park, Drs. Beckett and Pollack from Baltimore County, and Drs. Bryan, Eisenstein (PI), Mariuzza, McKenney and Polijak from CARB. This analytical ultracentrifuge will be used in our ongoing studies that are focussed on the determination of the quaternary structures of complex soluble and membrane associated proteins, the evaluation of the energetics of self- and heterologous-associations of proteins, the estimation of the strength of DNA-protein interactions, and the measurement of conformation changes promoted by small molecule binding to their macromolecular targets. The newly designed state-of-the-art Optima XL-A analytical ultracentrifuge that we are requesting, which offers a new level of precision and sensitivity in detecting the distribution of a solute under the influence of a centrifugal field, would considerably expand the scope of problems we could address by overcoming many of the limitations of our now obsolete Model E. The most vastly improved features of the Optima XL-A relative to the Model E include stable rotor drives, accurate and precise temperature control, powerful yet user-friendly system software enabling complete computer control over run conditions and data collection, and a vacuum enclosed monochromator that provides the capability to detect nanogram quantities of proteins by scanning absorbance down to 190 nm. Furthermore, the acquisition of a new analytical ultracentrifuge is essential due to the frequent equipment failure associated with keeping a 40 year old instrument running, and because Beckman will no longer service our Model E after June 1994.

来自三个校区的九位结构生物学家组成的主要用户组 在马里兰州系统的大学寻求资金的分析 ultracentrange将取代我们40年的E型ultracentrange， 碳水化合物。 主要用户群包括来自英国的Armstrong博士和Julin博士。 学院公园，贝克特博士和波拉克从巴尔的摩县，和博士。 布赖恩，爱森斯坦（PI），Mariuzza，McKenney和Polijak从CARB。 这 我们正在进行的研究中将使用分析性超声波， 重点研究了配合物的四级结构 可溶性和膜相关蛋白质，评价 蛋白质自缔合和异缔合的能量学， DNA-蛋白质相互作用强度的估计， 小分子结合促进的构象变化的测量 它们的大分子目标。 新设计的最先进的 Optima XL-我们要求的一种分析型超浓缩仪， 提供了一个新的水平的精度和灵敏度检测 在离心场的影响下溶质的分布， 将大大扩大我们可以解决的问题的范围， 克服了我们现在已经过时的E型车的许多局限性。 最 Optima XL-A相对于Model E的显著改进功能 包括稳定转子驱动器、精确的温度控制 功能强大但用户友好的系统软件， 控制运行条件和数据收集，以及真空封闭 提供检测纳克量的能力的单色仪 通过扫描吸收到190 nm的蛋白质。 而且 获得一种新的分析超浓缩物是必不可少的，因为 频繁的设备故障与保持40岁的 仪器运行，并且由于Beckman将不再为我们的模型提供服务， 1994年6月以后。