INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
基本信息
- 批准号:3756464
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:DNA binding protein DNA directed RNA polymerase DNA replication DNA replication origin Escherichia coli bacterial DNA bacterial proteins cell cycle gel mobility shift assay gene expression genetic promoter element genetic regulation genetic regulatory element genetic techniques genetic transcription microorganism growth protein structure function receptor coupling site directed mutagenesis
项目摘要
The long term goal of this project is to understand how initiation of DNA
replication is coupled to growth rate. Our objective is to understand
how DNA replication, cell growth, and cell division are so precisely and
coordinately regulated. In both prokaryotic and eukaryotic cells, the
rate of chromosomal duplication is controlled by the rate of initiation
at the origin(s) of replication, not by the rate of chain elongation.
The rate-limiting steps of the initiation event remain unidentified. To
understand how the frequency of the initiation event is determined, the
mechanisms that regulate the expression of the DnaA protein must be
understood. Both genetics and biochemistry are used to elucidate the
regulatory circuits controlling expression of the dnaA operon promoters.
The major dnaA promoter is growth rate regulated and under stringent
control. Guanosine 3',5'-bispyrophosphate(ppGpp) may provide the
molecular basis for growth rate regulation of DnaA protein expression.
Mutations in this promoter and in the dnaA box nearby that eliminate
promoter activity and binding of DnaA protein to the dnaA box will be
introduced into the chromosome and assayed for growth rate regulation and
stringent control in order to identify which regulatory sequences affect
growth rate regulation and stringent control. A single round in vitro
transcription assay is being used to ascertain the contribution of ppGpp
and DnaA protein to regulation of the activity of each of the dnaA
promoters. Mutants defective in the inhibitory response to ppGpp will
be examined for growth rate regulation and precise timing of initiation
of DNA replication in the cell cycle. We will determine whether the dnaA
gene is still under growth rate control in cells that lack ppGpp -- is
there a growth rate regulation mechanism other than ppGpp that controls
the expression of DnaA protein?
As in the past, MBRS students will be involved in both the technical and
scientific aspects of the research.
这个项目的长期目标是了解DNA是如何起始的
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JUDITH W ZYSKIND其他文献
JUDITH W ZYSKIND的其他文献
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{{ truncateString('JUDITH W ZYSKIND', 18)}}的其他基金
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
6576902 - 财政年份:2002
- 资助金额:
-- - 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
6435879 - 财政年份:2001
- 资助金额:
-- - 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
6301823 - 财政年份:2000
- 资助金额:
-- - 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
6107960 - 财政年份:1999
- 资助金额:
-- - 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
6107585 - 财政年份:1998
- 资助金额:
-- - 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
6240492 - 财政年份:1997
- 资助金额:
-- - 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
3778401 - 财政年份:
- 资助金额:
-- - 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
3857015 - 财政年份:
- 资助金额:
-- - 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
- 批准号:
3842168 - 财政年份:
- 资助金额:
-- - 项目类别:
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