INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE

DNA复制的起始--DNAA、RNA聚合酶、

基本信息

  • 批准号:
    3756464
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
  • 资助国家:
    美国
  • 起止时间:
  • 项目状态:
    未结题

项目摘要

The long term goal of this project is to understand how initiation of DNA replication is coupled to growth rate. Our objective is to understand how DNA replication, cell growth, and cell division are so precisely and coordinately regulated. In both prokaryotic and eukaryotic cells, the rate of chromosomal duplication is controlled by the rate of initiation at the origin(s) of replication, not by the rate of chain elongation. The rate-limiting steps of the initiation event remain unidentified. To understand how the frequency of the initiation event is determined, the mechanisms that regulate the expression of the DnaA protein must be understood. Both genetics and biochemistry are used to elucidate the regulatory circuits controlling expression of the dnaA operon promoters. The major dnaA promoter is growth rate regulated and under stringent control. Guanosine 3',5'-bispyrophosphate(ppGpp) may provide the molecular basis for growth rate regulation of DnaA protein expression. Mutations in this promoter and in the dnaA box nearby that eliminate promoter activity and binding of DnaA protein to the dnaA box will be introduced into the chromosome and assayed for growth rate regulation and stringent control in order to identify which regulatory sequences affect growth rate regulation and stringent control. A single round in vitro transcription assay is being used to ascertain the contribution of ppGpp and DnaA protein to regulation of the activity of each of the dnaA promoters. Mutants defective in the inhibitory response to ppGpp will be examined for growth rate regulation and precise timing of initiation of DNA replication in the cell cycle. We will determine whether the dnaA gene is still under growth rate control in cells that lack ppGpp -- is there a growth rate regulation mechanism other than ppGpp that controls the expression of DnaA protein? As in the past, MBRS students will be involved in both the technical and scientific aspects of the research.
这个项目的长期目标是了解DNA是如何起始的

项目成果

期刊论文数量(0)
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JUDITH W ZYSKIND其他文献

JUDITH W ZYSKIND的其他文献

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{{ truncateString('JUDITH W ZYSKIND', 18)}}的其他基金

INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    6576902
  • 财政年份:
    2002
  • 资助金额:
    --
  • 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    6435879
  • 财政年份:
    2001
  • 资助金额:
    --
  • 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    6301823
  • 财政年份:
    2000
  • 资助金额:
    --
  • 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    6107960
  • 财政年份:
    1999
  • 资助金额:
    --
  • 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    6107585
  • 财政年份:
    1998
  • 资助金额:
    --
  • 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    6240492
  • 财政年份:
    1997
  • 资助金额:
    --
  • 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    3778401
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    3857015
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
INITIATION OF DNA REPLICATION--DNAA, RNA POLYMERASE, & COUPLING TO CELL CYCLE
DNA复制的起始--DNAA、RNA聚合酶、
  • 批准号:
    3842168
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:

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  • 批准号:
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  • 财政年份:
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TFIIB 和 TFIIF 在 DNA 指导的 RNA 聚合酶 II 转录中的作用
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  • 财政年份:
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  • 资助金额:
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