NPBactID - Differential binding of peptoid functionalized nanoparticles to bacteria for identifying specific strains

NPBactID - 类肽功能化纳米粒子与细菌的差异结合，用于识别特定菌株

• 批准号: EP/Y029542/1
• 负责人: Aaron Lau
• 金额: $ 23.84万
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FY029542%2F1
• 关键词: NPBactID; Differential; binding; peptoid; functionalized

A rising public health crisis is antimicrobial resistance (AMR). A key mitigation strategy is to prescribe only the most appropriate antibiotics to reduce the off-targeting that leads to resistance. However, current methods to identify the cause of infection are generally laboratory-based and slow. Convenient, synthetic strain-specific affinity probes (SAPs) could in principle enable rapid, point- of-care biosensors but these are lacking in particular for identifying different strains of bacteria. This project will investigate a novel approach for developing SAPs by exploiting recent results from the host group showing that varying the functionalization directions and densities of antimicrobial "peptoids" immobilized on material surfaces can generate large differences in the surface attachment of different bacteria. Combined with the expertise of the postdoctoral researcher in nanoparticle (NP) synthesis and surface functionalization, this project will develop peptoid functionalized NPs as a novel SAP platform that can generate unique signals when the particles bind to different bacterial strains. We envision that this principle of differential surface functionalization combined with NP encoding can be adapted to a range of biosensing approaches, and thus open a new horizon in bacterial identification to help combat AMR.

一个日益严重的公共卫生危机是抗菌素耐药性（AMR）。一个关键的缓解策略是只开最合适的抗生素，以减少导致耐药性的脱靶。然而，目前确定感染原因的方法通常是基于实验室的，而且速度缓慢。方便的合成菌株特异性亲和探针（SAP）原则上可以实现快速的即时生物传感器，但这些探针特别缺乏用于鉴定不同菌株的细菌。该项目将研究一种开发SAP的新方法，方法是利用宿主组的最新结果，这些结果表明，固定在材料表面上的抗菌“类肽”的功能化方向和密度的变化可以在不同细菌的表面附着上产生很大的差异。结合博士后研究人员在纳米颗粒（NP）合成和表面功能化方面的专业知识，该项目将开发类肽功能化的NP作为一种新型SAP平台，当颗粒与不同的细菌菌株结合时，可以产生独特的信号。我们设想，这种差异表面功能化与NP编码相结合的原理可以适用于一系列生物传感方法，从而为细菌鉴定开辟了新的视野，以帮助对抗AMR。