STUDIES OF HUMAN T-HELPER CELL TYPE 1 AND TYPE 2 CYTOKINE RESPONSES

辅助性T细胞已被分为不同的亚群， 它们分泌的细胞因子。 TH 0细胞分泌IL 2、IL 4和IFN γ; TH 1细胞 分泌IL 2和IFN γ;和TH 2细胞分泌IL 4。 易感性 某些传染病已被证明是依赖于 主要类型的TH细胞诱导。 有一些证据表明 HIV-1感染的进展与偏向TH 2细胞有关。 因此，如果HIV-1感染的疫苗诱导抗- HIV免疫通过募集TH 1而不是TH 2细胞。 B。流产一直是 在小鼠中诱导TH 1细胞。 从B开始。流产也会刺激 人类B细胞在T-独立的方式，我们一直在研究它作为一个 作为疫苗的HIV-1蛋白和肽的可能载体。 这是 因此感兴趣的是确定什么效果B。流产会对 人类T细胞 通过外周血传代纯化人T细胞， 单核细胞在尼龙棉上，然后用梯度洗脱 离心法 小的静息T细胞在以下存在下被刺激： B。流产，并显示分泌IFN γ和IL 2，但不分泌IL 4。 同样地， 从这些细胞中PCR扩增的RNA显示B。人工流产 IFN γ、IL 2和IL 2 R mRNA，但无IL 4 mRNA。 IFN γ的来源 不是来自污染的NK细胞，因为磁珠去除NK细胞 细胞对结果没有影响，富集的NK细胞耗尽了T细胞， 细胞对B无反应。流产 这些结果表明，B. 流产病毒可以刺激来自人T细胞的TH 1应答，因此可能是 作为HIV-1感染者的载体是有益的。

T helper cells have been divided into different subsets based on the cytokines they secrete. THO cells secrete IL2, IL4 and IFNgamma; TH1 cells secrete IL2 and IFNgamma; and TH2 cells secrete IL4. Susceptibility to certain infectious diseases has been shown to be dependent on the predominant type of TH cell induced. There is some evidence that progression of HIV-1 infection is associated with a bias towards TH2 cells. Thus, vaccines for HIV-1 infection may be advantageous if they induce anti- HIV immunity by recruiting TH1 rather than TH2 cells. B. abortus has been shown to induce TH1 cells in mice. Since B. abortus can also stimulate human B cells in a T-independent manner we have been studying it as a possible carrier for HIV-1 proteins and peptides as a vaccine. It was therefore of interest to determine what effect B. abortus would have on human T cells. Human T cells were purified by passage of peripheral blood mononuclear cells over nylon wool followed by percoll gradient centrifugation. Small resting T cells were stimulated in the presence of B. abortus and shown to secrete IFNgamma and IL2, but not IL4. Similarly, PCR amplification of RNA from these cells showed that B. abortus induced IFNgamma, IL2 and IL2R mRNA but no IL4 mRNA. The source of the IFNgamma was not from contaminating NK cells because magnetic bead removal of NK cells had no effect on the outcome, and enriched NK cells depleted of T cells did not respond to B. abortus. These results indicate that B. abortus can stimulate TH1 responses from human T cells, and as such may be beneficial as a carrier for persons infected with HIV-1.