IL-4-INDUCED SIGNAL TRANSDUCTION PATHWAYS IN HEMATOPOIETIC CELLS

IL-4 诱导的造血细胞信号转导途径

• 批准号: 3774920
• 负责人: J H PIERCE
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774920
• 关键词: biological signal transduction; enzyme substrate; human tissue; insulin; interleukin 3; interleukin 4; mitogens; myeloid stem cell; phosphorylation; protein tyrosine kinase; tissue /cell culture

Interleukin 4 (IL-4) is a member of a cytokine superfamily that exerts multiple effects on cells of hematopoietic origin. It was demonstrated that IL-4 induces a potent mitogenic response but not long-term growth in the IL-3-dependent myeloid progenitor cell line, FDC-P2. IL-4 and IL- 3 were shown to activate distinct tyrosine phosphorylation cascades in the same FDC-P2 cell background. This may reflect a difference in the biological function of these two cytokines. Insulin also induces a potent mitogenic response but not long-term growth in FDC-P2 cells. Test results demonstrated that IL-4 and insulin induced striking tyrosine phosphorylation of some 170 kD substrates, termed 4PS, in FDC-P2 cells, suggesting that these two factors share overlapping signaling pathways in hematopoietic cells.

白细胞介素4（IL-4）是细胞因子超家族的一员，其发挥 对造血来源细胞的多重影响。 结果表明 IL-4诱导有效的促有丝分裂反应，但不诱导长期生长， IL-3依赖性骨髓祖细胞系FDC-P2。 IL-4和IL- 3显示激活不同的酪氨酸磷酸化级联反应， 相同的FDC-P2细胞背景 这可能反映了 这两种细胞因子的生物学功能。 胰岛素也诱导一种强有力的促有丝分裂反应，但不能诱导长期生长 在FDC-P2细胞中。 实验结果表明，IL-4和胰岛素诱导 一些170 kD底物的显著酪氨酸磷酸化，称为4PS， 在FDC-P2细胞中，这表明这两个因子有重叠 造血细胞中的信号通路。