MOLECULAR BIOLOGY OF NEURAL FUNCTION

下面的摘要描述了五个试图调查的项目， 在分子水平上，作用于许多分子，包括，A 激酶、运动蛋白轻链、调节神经肽Buccalin和 肌调节蛋白、Rab 3和一种被认为调节CDC~2~样蛋白的新蛋白 仅存在于神经元中的蛋白质，在神经功能中发挥作用。我们有 鉴定海洋中的水囊素和肌调节蛋白的多肽家族 Hare Calfornica，试图获得关于如何 多肽家族共同参与突触前或突触后 神经调节。多肽受体的分子表征 通过它信号转导级联被激活以影响 调节，例如G蛋白偶联受体及其各自的 A蛋白激酶的调节也在积极进行中。一家人 已经在神经系统中发现了运动蛋白轻链转录本 乌贼洛戈·佩雷。蛋白质的过度表达和纯化是 目前被用作确定功能和特异性的手段 因为它们与细胞内运输，特别是轴突运输有关 神经元中膜结合细胞器的数量。小的突触 囊泡特异的GTP结合蛋白Rab 3a已从 乌贼视叶。利用任一整体的显微注射实验 过表达的Rab 3a和Rab 3a多肽的蛋白质或片段 抗体将被用来帮助确定Rab 3a在 突触小泡在突触前膜停靠和/或融合。一个 第五种蛋白质，p62，被认为调节一种 神经丝特异的CDC~2蛋白激酶样活性已被克隆 并从大鼠脑cDNA文库中鉴定了其特征。

The following summary describes five projects that attempt to investigate, at the molecular level, the roles a number of molecules, including, A kinase, kinesin light chains, the modulatory neuropeptides buccalin and myomodulin, rab 3 and a novel protein thought to regulate a cdc~2~like protein found exclusively in neurons, play in neural function. We have characterized the peptide families of buccalin and myomodulin from the sea hare Aplysia californica in an attempt to gain further insights as to how peptide families collectively contribute to pre~ or postsynaptic neuromodulation. The molecular characterization of peptide receptors through which signal transduction cascades are activated to affect modulation, for example G protein~coupled receptors, and their respective regulation by A kinases is also being actively pursued. A family of kinesin light chain transcripts have been identified in the nervous system of the squid Loligo pealei. Protein over~expression and purification is currently being employed as a means to determine function and specificity as they relate to intracellular transport, particularly axonal transport of membrane bound organelles in neurons. The small, synaptic vesicle~specific, GTP~binding protein, rab 3a, has been cloned from the squid optic lobe. Microinjection experiments utilizing either whole protein or fragments of over~expressed rab 3a, and rab 3a peptide~specific antibodies will be used to help determine the role rab 3a plays in synaptic vesicle docking and or fusion at the presynaptic membrane. A fifth protein, p62, thought to regulate the activity of a neurofilament~specific cdc~2 protein kinase~like activity has been cloned and characterized from a rat brain cDNA library.