REGULATION OF MUCOSAL IMMUNE RESPONSES IN HUMANS AND NON-HUMAN PRIMATES

人类和非人类灵长类动物粘膜免疫反应的调节

• 批准号: 3790755
• 负责人: W STROBER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790755
• 关键词: B lymphocyte; CD3 molecule; Primates; SDS polyacrylamide gel electrophoresis; T cell receptor; T lymphocyte; human subject; immunoglobulin structure; immunoprecipitation; immunoregulation; leukocyte activation /transformation; leukocyte adhesion molecules; lymph nodes; lymphocyte proliferation; tissue /cell culture; western blottings

In these studies we are defining the role of the LAM-1 adhesion (MEL-14 lymph node homing receptor) molecule in T cell and B cell function. In preliminary studies, we determined that anti-LAM-1 antibodies can augment anti-CD3-induced proliferation of purified T cells. In further studies we immunoprecipitated surface radioiodinated T cell lysates with anti-CD3 and anti-LAM-1 mAb to determine if molecules in the TCR/CD3 complex associate with Leu 8 molecules. Although Leu 8 mAb immunoprecipitated only a single protein of approximately 80 kDa from T cell lysates treated with Nonidet P-40 under reducing condition, it coimmunoprecipitated additional proteins of 48, 42, 28, 24, and 22 kDa from T cell lysates treated with 3-[(3- cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS). These additional proteins were identified as the alpha-, beta-, gamma-, delta-, and epsilon-chains of the TCR/CD3 complex by one-dimensional and two- dimensional diagonal SDS-PAGE. Densitometric scanning showed that, on average, 18% of the TCR/CD3 complex associates with LAM-1. In a final study, we showed by immunoblotting anlaysis using anti- peptide antibody that anti-LAM-1 mAb coimmunoprecipitates the chain of CD3. These results indicate that the human-lymph node homing receptor homologue (LAM- 1) participates in the activation of T cells, probably via its association with the TCR/CD3 complex.

在这些研究中，我们正在定义LAM-1黏附(MEL-14)的作用 淋巴归巢受体)分子在T细胞和B细胞中的功能。在……里面 初步研究，我们确定抗LAM-1抗体可以增强 抗CD3抗体诱导纯化的T细胞增殖。在进一步的研究中，我们 免疫共沉淀表面放射性碘标记T细胞裂解物与抗CD3和 抗LAM-1单抗用于确定TCR/CD3复合体中的分子是否相关 用亮氨酸8分子。尽管Leu8单抗只免疫沉淀一次 诺奈特处理的T细胞裂解物中约80 kDa的蛋白质 P-40在还原条件下，免疫共沉淀额外的蛋白质 经3-[(3-)]处理的48、42、28、24和22 kDa的T细胞裂解物 Cholamidopropyl)-dimethylammonio]-1-propanesulfonate(CHAPS)。这些 其他蛋白质被鉴定为α-，β-，伽马-，三角洲-， 以及TCR/CD3复合体的epsilon链的一维和二维结构. 维度对角线十二烷基硫酸钠-PAGE。密度扫描显示，在 平均而言，18%的TCR/CD3复合体与LAM-1相关。在决赛中 研究表明，我们使用抗肽抗体进行了免疫印迹分析 抗LAM-1单抗免疫共沉淀CD3链。这些 结果表明，人-淋巴归巢受体同源物(LAM-1)与人淋巴结归巢受体同源物(LAM-1)有关。 1)参与T细胞的激活，可能是通过它的关联 与TCR/CD3复合体结合。