REGULATION OF MUCOSAL IMMUNE RESPONSES IN HUMANS AND NON-HUMAN PRIMATES

在这些研究中，我们定义了LAM-1粘附（MEL-14）的作用 淋巴结归巢受体）分子在T细胞和B细胞功能中作用。 在 初步研究，我们确定抗LAM-1抗体可以增加 抗CD 3诱导的纯化T细胞增殖。 在进一步的研究中，我们 用抗CD 3免疫沉淀表面放射性碘标记的T细胞裂解物， 抗LAM-1 mAb，以确定TCR/CD 3复合物中的分子是否与 Leu 8分子 虽然Leu 8 mAb仅免疫沉淀了单个 来自用Nonidet处理的T细胞裂解物的约80 kDa的蛋白 P-40在还原条件下，它共免疫沉淀额外的蛋白质 48、42、28、24和22 kDa的T细胞裂解物中，用3-[（3-甲基-IH-吡唑-4-基）-N-甲基-IH-吡唑-4-基]-N-甲基-N-吡唑-4-基]-酮处理。 胆酰胺丙基）-二甲基铵基]-1-丙磺酸盐（CHAPS）。 这些 其它蛋白质被鉴定为α-，β-，γ-，δ-， 和ε-链的TCR/CD 3复合物通过一维和二维- 三维对角线SDS-PAGE。 密度扫描显示，在 平均18%的TCR/CD 3复合物与LAM-1结合。 在最终 研究中，我们用抗- 肽抗体 抗LAM-1单克隆抗体共免疫沉淀 CD 3链。 这些 结果表明，人淋巴结归巢受体同源物（LAM- 1)参与T细胞的活化，可能是通过其关联 TCR/CD 3复合物。

In these studies we are defining the role of the LAM-1 adhesion (MEL-14 lymph node homing receptor) molecule in T cell and B cell function. In preliminary studies, we determined that anti-LAM-1 antibodies can augment anti-CD3-induced proliferation of purified T cells. In further studies we immunoprecipitated surface radioiodinated T cell lysates with anti-CD3 and anti-LAM-1 mAb to determine if molecules in the TCR/CD3 complex associate with Leu 8 molecules. Although Leu 8 mAb immunoprecipitated only a single protein of approximately 80 kDa from T cell lysates treated with Nonidet P-40 under reducing condition, it coimmunoprecipitated additional proteins of 48, 42, 28, 24, and 22 kDa from T cell lysates treated with 3-[(3- cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS). These additional proteins were identified as the alpha-, beta-, gamma-, delta-, and epsilon-chains of the TCR/CD3 complex by one-dimensional and two- dimensional diagonal SDS-PAGE. Densitometric scanning showed that, on average, 18% of the TCR/CD3 complex associates with LAM-1. In a final study, we showed by immunoblotting anlaysis using anti- peptide antibody that anti-LAM-1 mAb coimmunoprecipitates the chain of CD3. These results indicate that the human-lymph node homing receptor homologue (LAM- 1) participates in the activation of T cells, probably via its association with the TCR/CD3 complex.