PHOTODYNAMIC THERAPY--BASIC SCIENCE STUDIES

在这个竞争的延续应用程序中，我们建议扩展我们的 光动力疗法(PDT)的研究其中三项建议 项目的目的是阐明光动力疗法引起 用于癌症治疗时的细胞/组织反应。每个人都在后面 一种截然不同的方法来实现其目标；在随后的几年中， 每个项目都与其他项目进行交互。有一个项目特别涉及 用非侵入性方法最终提高光动力疗法和一种 随着基础实验室数据扩展到原发肿瘤， 兽医临床试验。 具体地说，本提案中的项目建议： 1)探讨光动力疗法与热疗的相互作用机制； 2)将实验室模型中的结果推广到自发兽医 肿瘤； 3)建立PDT耐药肿瘤模型，并随后阐明 参与这种抗性的机制； 4)开发组织光敏剂的非侵入性定量方法 浓度；以及 5)建立对真实组织光敏性的可量化测量 允许各种光动力疗法的相互比较。 这项计划是一项多学科、多机构的调查 目的是了解和改进光动力疗法在癌症治疗中的应用 癌症。组成项目之间的相互关系增强了 全力以赴。

In this competing continuation application we propose to extend our investigation of Photodynamic Therapy (PDT). Three of the proposed projects are directed at elucidating the mechanisms by which PDT elicits cellular/tissue response when employed in cancer treatment. Each follows a distinctly different approach to achieve its goals; in subsequent years, each interacts with the other projects. One project deals specifically with non invasive methods to ultimately improve the utility of PDT and one with the extension of fundamental laboratory data into a primary tumor, veterinary clinical trial. Specifically, the projects in this proposal propose to: 1) examine the mechanisms of interaction between PDT and hyperthermia; 2) extend results obtained in laboratory models to spontaneous veterinary tumors; 3) develop a PDT resistant tumor model and subsequently elucidate the mechanisms involved in such resistance; 4) develop non invasive methods to quantitate tissue photosensitizer concentrations; and 5) to establish a quantifiable measure of true tissue photosensitivity to allow the intercomparison of various PDT treatments. This program is a multidisciplinary, multi-institutional investigation aimed at understanding and improving the use of PDT for the treatment of cancer. The inter-relation between the constituent projects enhances the overall effort.