单核细胞分化——酪氨酸磷酸化通过 CSF-1 受体的作用-猫眼课题宝

单核细胞分化——酪氨酸磷酸化通过 CSF-1 受体的作用

批准号：

3792629

负责人：

J PURI

金额：

$0.0万

依托单位：

CENTER BIOLOGICS EVALUATION RESEARCH HEMATOLOGY

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/3792629

关键词：

arachidonate cell differentiation cell growth regulation colony stimulating factor eicosanoid metabolism enzyme activity enzyme inhibitors growth factor receptors hematopoietic stem cells monocyte phosphorylation prostaglandin E prostaglandin endoperoxide synthase protein tyrosine kinase tissue /cell culture transfection

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英文摘要

The mouse hematopoietic cell ine, 32D, was transfected with c-fms, which enclodes for the CSF-1 receptor, a tyrosine kinase (TK). In the absence of CSF-1, transfected cells show moderate levels of arachidonic acid (A.A.) release and produce a substantial amount of prostaglandin E2 (PGE2) in comparison with the original cell line. Exposure of transfected cells to CSF-1, while inducing a substantial increase in arachidonate release, nevertheless resulted in inhibition of PGE2 production. Addition of ST638, a tyrosine kinase inhibitor, to cells transfected with c-fms in the absense of CSF-1 inhibited PGE2 within 10 to 60 minutes. Its addtion to the same cells in the presence of CSF-1 receptor may regulate cyclooxygenase activity. The different effect of CSF-1 receptor of PGE2 production in the presence or absence of CSF-1 and the opposite effect of a tyrosine kinase inhibitor on PGE2 suggest that both the receptor alone or the receptor-ligand complex transduce on active, but different, signal through tyrosine phosphorylation.

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