ONCOIMMUNIN--NEW TUMOR-DERIVED IMMUNOREGULATORY FACTORS

两种新的细胞因子被命名为肿瘤免疫蛋白-L(Mw 45kD) 和肿瘤性免疫蛋白-M(Mw 36kD)鉴定和纯化为均一 通过对肿瘤细胞上清液进行SDS-PAGE分析。为了实现这一目标， 离子交换，凝胶过滤，疏水作用， 进行了反相色谱分离。 肿瘤免疫球蛋白-L是人T淋巴细胞有丝分裂原，肿瘤免疫球蛋白-M对其抑制作用 至少三个人髓系白血病细胞系的生长。 伴随着抑制增殖，后一种因素 诱导细胞表面整合素CD11的表达和细胞迁移 细胞走向趋化梯度。 该项目的结果旨在探索可能的 肿瘤起源的可溶性免疫调节因子的存在支持 认为免疫细胞识别的肿瘤免疫环境是 除了经过充分研究的细胞-细胞外，还由可溶介质定义 联系互动。

Two new cytokines which have been designated as Oncoimmunin-L (MW 45kD) and Oncoimmunin-M (MW 36 kD) were identified and purified to homogeneity by SDS-PAGE analysis from a tumor cell supernatant. To achieve this end, combinations of ion exchange, gel filtration, hydrophobic interaction, and reverse phase chromatography were implemented. Oncoimmunin-L is a human T-lymphocyte mitogen and Oncoimmunin-M inhibits the growth of at least three human myeloid leukemic cell lines. Concomitantly with the inhibition of proliferation, the latter factor induces the cell surface expression of the integrin CD11 and migration of cells toward a chemotactic gradient. Results from this project which was aimed at exploring the possible presence of soluble immunoregulatory factors of tumor origin support the idea that the tumor immunoenvironment as recognized by immunocytes is defined by soluble mediators in addition to the well-studied cell-cell contact interactions.