ONCOIMMUNIN--NEW TUMOR-DERIVED IMMUNOREGULATORY FACTORS

癌免疫素--新的肿瘤源性免​​疫调节因子

• 批准号: 3792445
• 负责人: B PACKARD
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792445
• 关键词: CD antigens; SDS polyacrylamide gel electrophoresis; T lymphocyte; cell growth regulation; cell migration; cytokine; gel filtration chromatography; growth factor; growth inhibitors; integrins; ion exchange chromatography; leukocyte adhesion molecules; lymphocyte proliferation; mitogens; neoplasm /cancer immunology; protein purification; reversed phase chromatography

Two new cytokines which have been designated as Oncoimmunin-L (MW 45kD) and Oncoimmunin-M (MW 36 kD) were identified and purified to homogeneity by SDS-PAGE analysis from a tumor cell supernatant. To achieve this end, combinations of ion exchange, gel filtration, hydrophobic interaction, and reverse phase chromatography were implemented. Oncoimmunin-L is a human T-lymphocyte mitogen and Oncoimmunin-M inhibits the growth of at least three human myeloid leukemic cell lines. Concomitantly with the inhibition of proliferation, the latter factor induces the cell surface expression of the integrin CD11 and migration of cells toward a chemotactic gradient. Results from this project which was aimed at exploring the possible presence of soluble immunoregulatory factors of tumor origin support the idea that the tumor immunoenvironment as recognized by immunocytes is defined by soluble mediators in addition to the well-studied cell-cell contact interactions.

两种新的细胞因子被命名为Oncoimmunin-L（分子量45 kD） 和Oncoimmunin-M（分子量36 kD）进行了鉴定和纯化， 通过肿瘤细胞上清液的SDS-PAGE分析。 为了达到这个目的， 离子交换，凝胶过滤，疏水相互作用， 和反相色谱。 肿瘤免疫素-L是人T淋巴细胞有丝分裂原，并且肿瘤免疫素-M抑制 至少三种人类骨髓性白血病细胞系的生长。 伴随着增殖的抑制，后一个因素 诱导整合素CD 11的细胞表面表达和 细胞趋化梯度。 该项目旨在探索可能的 肿瘤来源的可溶性免疫调节因子的存在支持了 认为免疫细胞识别的肿瘤免疫环境 由可溶性介质定义，除了研究充分的细胞-细胞 接触互动。