HISTOPATHOLOGY AND TOXICITY OF PERTUSSIS HEAT LIABLE TOXIN

百日咳热不稳定毒素（PEHLT）是百日咳杆菌产生的几种毒素之一。 百日咳杆菌。 的生物化学和药理学作用 PHELT及其与人类临床百日咳的关系尚不清楚。 PEHLT在皮下注射时表现出毒性活性， 腹膜内或静脉内注射到啮齿动物体内。 我们检查了 高纯度PHELT制剂的组织病理学特征 对小鼠 将高度纯化的PEHLT（200 μ g）皮下注射至 新生小鼠引起早期急性炎症反应， 浅表皮下组织和真皮下层， 血管周围单核细胞浸润，血管充血，真皮 水肿，红细胞外渗，最后出血。 注射 将毒素腹膜内注射到18-20 gm NIH通用小鼠中 导致胸腺退化和脾淋巴细胞的剂量依赖性减少 尺寸 脾脏大小的变化与组织学改变有关。 红系和髓系前体细胞坏死 纸浆 在亚致死剂量下，PHELT还引起红细胞耗竭， 骨髓中的髓样成分。

Pertussis heat-labile toxin (PEHLT) is one of several toxins produced by Bordetalla pertussis. The biochemical and pharmacologic effects of PHELT and its relationship to clinical pertussis in humans is not known. PEHLT exhibits toxic activities when injected subcutaneously, intraperitoneally, or intravenously into rodents. We examined the gross and histophathologic features of a highly purified preparation of PHELT in mice. Highly purified PEHLT (200 pg) injected subcutaneously into newborn mice elicited an early acute inflammatory resonse in the superficial subcutaneous tissues and lower dermis followed by a mononuclear cell perivascular infliltrate, vascular congestion, dermal edema, extravasation of erythrocytes, and finally hemorrhage. Injection of the toxin intraperitoneally into 18-20 gm NIH general purpose mice resulted in thymic involution and a dose dependent decrease in splenic size. The changes in splenic size correlated with the histologic findings of necrosis of erythroid and myeloid precursors in the red pulp. At sublethal doses, PHELT also caused depletion of erythroid and myeloid elements in the bone marrow.