IMMUNOGENICITY OF PNEUMOCOCCAL GROUP 9 POLYSACCHARIDE-PROTEIN COMPLEX

了解多糖(PS)对免疫应答的调节作用 并试图开发一种更有效的预防疫苗 对于幼儿肺炎球菌疾病，我们研究了免疫反应 9V PS-破伤风类毒素(TT)结合物对幼龄小鼠的影响 母体免疫对幼鼠对9VPS-TT免疫应答的影响。 2周龄幼鼠产生抗9VPS或PS蛋白的IgM抗体 共轭。然而，仅有9VPS-TT结合物诱导产生一种抗体。 当第二次免疫原注射时的反应和无名效应 每隔2周注射一次。氢氧化铝凝胶增强免疫球蛋白的研究 对9VPS-TT的抗体应答不能增强对9VPS的应答。这个 Gel也有助于同型从IgM到Ig G的转换。 孕前和/或孕期母体免疫使幼鼠 对9VPS-TT结合免疫原有效应答。注射怀孕 免疫结合物的小鼠增强免疫球蛋白G抗体应答 9VPS-TT在子代。这些结果表明，小鼠的免疫接种 在孕前和/或孕期注射9VPS-TT免疫原最佳剂量的母亲 在怀孕和幼儿中使用PS蛋白结合物可以提供 对肺炎球菌PSS抗体反应增强的幼儿。

To understand the modulation of immune response to polysaccharide (PS) immunogens and attempt to develop a more effective vaccine for prevention of pneumococcal diseases in young children, we studied the immune response of young mice to a type 9V PS-tetanus toxoid (TT) conjugate and the effect of maternal immunization on the immune response of young mice to 9VPS-TT. Young mice at 2 weeks of age produced IgM antibody to 9VPS or PS-protein conjugate. However, only 9VPS-TT conjugate induced an IgG antibody response and an anamestic effect when a second dose of the immunogen was injected at 2 weeks interval. Aluminum hydroxide gel enhanced the IgG antibody response to 9VPS-TT but did not augment the response to 9VPS. The gel also helped in the isotype switching from IgM to IgG. Maternal immunization before and/or during gestation primed young mice to respond effectively to 9VPS-TT conjugate immunogen. Injection of pregnant mice with the conjugate immunogen enhanced the IgG antibody response to 9VPS-TT in the offspring. These results indicated that immunization of mothers with an optimum dose of 9VPS-TT immunogen before and/or during pregnancy and in young children with a PS-protein conjugate can provide young children with an enhanced antibody response to pneumococcal PSs.