GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
批准号:
3796454
负责人:
M GOTTESMAN
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
P glycoprotein Retroviridae adenosinetriphosphatase antineoplastics bone marrow cis platinum compound complementary DNA disease /disorder model drug metabolism gastrointestinal drug absorption gene expression gene therapy genetic transduction genetically modified animals glycoproteins human tissue laboratory mouse leukopenia liposomes membrane transport proteins messenger RNA model design /development molecular cloning molecular genetics multidrug resistance mutant neoplasm /cancer chemotherapy neoplasm /cancer genetics neoplastic cell nucleic acid sequence phenotype protein purification transfection transfection /expression vector
中文摘要
对人类癌症的化疗耐药性进行了研究
建立模拟发育的体外和体内遗传系统
抗药性。人类多药耐药的主要机制
肿瘤是我们已经克隆和测序的mdr1基因的表达。
逆转录病毒载体携带的MDRI基因的表达可用于
将细胞转化为多药耐药,并已被用于授予
体外和体内对小鼠骨髓细胞的耐药性。机械论
对P-糖蛋白的研究表明,它与药物的清除有关
直接从质膜中提取。为了研究机制,我们已经生成了
许多突变体和启动了包括提纯在内的生化研究
P-糖蛋白与药物依赖性ATPase活性的重建
人工脂质体。
英文摘要
Resistance to chemotherapy of human cancers has been studied by
establishing in vitro and in vivo genetic systems which mimic development
of drug resistance. A major mechanism of multidrug resistance in human
cancer is expression of the MDR1 gene which we have cloned and sequenced.
Expression of the MDRI gene carried by retroviral vectors can be used to
transduce cells to multidrug resistance and has been used to confer
resistance to mouse bone marrow cells in vitro and in vivo. Mechanistic
studies on P-glycoprotein suggest that it is involved in removing drug
directly from the plasma membrane. To study mechanism, we have generated
many mutants and initiated biochemical studies including the purification
of P-glycoprotein and reconstruction of drugdependent ATPase activity in
artificial liposomes.
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GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
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批准号:3813347
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资助金额:$0.0万
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财政年份:--
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负责人:M GOTTESMAN
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依托单位:
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
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批准号:6160929
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资助金额:$0.0万
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财政年份:--
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负责人:M GOTTESMAN
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依托单位:
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
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批准号:2463652
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资助金额:$0.0万
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财政年份:--
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负责人:M GOTTESMAN
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依托单位:
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
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批准号:3774310
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资助金额:$0.0万
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财政年份:--
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负责人:M GOTTESMAN
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依托单位:
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
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批准号:3752025
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资助金额:$0.0万
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财政年份:--
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负责人:M GOTTESMAN
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依托单位:
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
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批准号:6100829
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资助金额:$0.0万
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财政年份:--
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负责人:M GOTTESMAN
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依托单位:
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
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批准号:5200938
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M GOTTESMAN
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