CHARACTERIZ. OF A NEW HUMAN PATHOGEN MYCOPLASMA ISOLA FROM AIDS PTS W K.SARC

特点。

• 批准号: 3874317
• 负责人: S C LO
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874317
• 关键词: AIDS; DNA; DNA virus; Kaposi's sarcoma; Mycoplasma; Pongidae; antibody; clone cells; diagnosis design /evaluation; human tissue; molecular cloning; opportunistic infections; secondary infection; serology /serodiagnosis; serotyping; tissue /cell culture; transfection; virus infection mechanism

A new strain of mycoplasma originated in transfection of a standard NIH-3T3 cell line with DNA isolated from AIDS patients' tissues was designated as Mycoplasma incognitus. This agent was found to be infectious in cell culture and in the monkey to cause a wasting syndrome and death. Specific genetic clones and antibodies were obtained for this agent and used to probe infected tissues. Attempts have been made to develop molecular and serological assays and to assess the significance of this newly identified agent in human disease. Genetic analysis of this agent by liquid hybridization procedure has shown that DNA of this agent closely resembles M. fermentans. M. fermentans has been associated with leukemia previously.

一株新的支原体来源于NIH-3 T3标准质粒的转染 具有从艾滋病患者组织中分离的DNA的细胞系被命名为 不明支原体发现该因子在细胞中具有感染性 在猴子体内引起消耗综合症和死亡。具体 获得了该试剂的遗传克隆和抗体，并用于 探测感染的组织已经尝试开发分子和 血清学检测，并评估这一新发现的意义 人类疾病的病原体。通过液体对该制剂进行遗传分析 杂交程序表明，该试剂DNA与 M.发酵剂。M.发酵素以前与白血病有关。