REQUIREMENTS FOR CLASS I RESTRICTED E CELL RECOGNITION OF ANTIGEN

虽然有数据表明 I 类和 II_restricted 抗原决定簇存在于短肽上，仅此而已 了解抗原加工和呈递的机制 对于 I 类抗原的限制性识别。 我们的初步 数据表明 I 类限制性溶细胞 T 细胞 (CTL) cAn 产生可溶性蛋白抗原卵清蛋白 (OVA) 抗原以 OVA 中肽的形式使用 胰蛋白酶消化，但用作完整蛋白质时则不然。 我们将使用 OVA，在处理和处理方面具有良好的特性 II 类限制性反应的呈现途径，其中 可以大量以纯形式提供，作为模型 研究抗原的加工和呈递 I 类分子的背景。 影响OVA诱导类能力的参数 将研究 I 限制性 CTL 体内活性，以便 确定抗原肽是否可以直接施用 动物或是否需要抗原“引发” 细胞。 我们将确定是否存在含有以下成分的抗原： “辅助决定因素”，可能与 I 类限制有关 决定因素，增强 CTL 启动。 我们将定义肽 在 OVA 内产生 I 类限制反应和 研究这些肽与以下物质形成复合物的能力 他们的I类限制元件。 最后，我们将调查 是否进行抗原处理以产生 I 类与 I 类 II 限制性决定因素涉及相同或不同的代谢 途径。

While there is data to suggest that both class I and II_restricted antigenic determinants are present on short peptides, little more is known about the mechanism of antigen processing and presentation for class I restricted recognition of antigen. Our preliminary data indicate that class I-restricted cytolytic T cells (CTL) cAn be generated to the soluble protein antigen ovalbumin (OVA) when the antigen is used in the form of peptides present in a OVA tryptic digest but not when used as an intact protein. We will use OVA, which is well characterized with respect to the processing and presentation pathway for class II restricted responses and which is available in large quantities in pure forms, as a model to investigate the processing and presentation of antigen in the context of class I molecules. The parameters that influences the ability of OVA to induce class I-restricted CTL activity in vivo will be investigated in order to determine whether antigenic peptides can be administered directly to the animal or whether there is a need for an antigen "priming" cell. We will determine if the presence of an antigen containing a "helper determinant", perhaps linked to the class I-restricted determinant, enhances CTL priming. We will define the peptides within OVA that generate class I restricted responses and investigate the capacity of these peptides to form complexes with their class I restriction elements. Finally, we will investigate whether antigen processing for the generation of class I vs. class II restricted determinants involve the same or different metabolic pathways.