IMMUNOCHEMICAL STUDIES OF ACTINOMYCES VISCOSUS POLYSACCHARIDES

粘性放线菌产生的多糖可能促进 这些微生物对吸附在糖蛋白上的黏附 牙釉质或裸露的牙根表面。它们可能会抑制或增强 凝集素介导的口腔细菌间的共聚集，以及 构成了斑块的大部分。这些多糖还可以 诱导形成可能阻碍或促进抗体的形成 龋齿的发展。虽然粘性酵母菌产生 几种多糖的化学精细结构保持到 将被澄清。它们在牙根发病机制中的作用评价 表层龋齿也缺乏，因此，这项建议的目的是： 1-在完整粘性支原体细胞表面的定量 荧光标记凝集素结合分析产生信息 关于结合部位的性质、数量和亲和力(Ka)； 2-以纯化的形式分离，细胞壁，相关的细胞壁和 鸡传染性支气管炎病毒T6、T14V和T14Av株的胞外多糖 粘性葡萄球菌的分子结构。 化学和免疫化学方法； 3-检测分离出的多糖作为 抗原抑制细菌在唾液涂层上的附着 羟基磷灰石包裹的珠子和抑制细菌共聚集； 4-检测大鼠结合位点特异性抗体和 兔多克隆抗体分离粘菌多糖的研究 抗原； 5-用分离的多糖抗原主动免疫大鼠 以确定特定聚合物刺激的能力 抑制或加强口腔感染时的疾病和 6-制备鼠抗分离株的单抗 多糖类抗原，阐明其结合部位的特异性 并评估特定的抗碳水化合物在修饰中的作用 无特定病原体大鼠的植入和疾病。

Polysaccharides produced by Actinomyces viscosus may promote adherence of those microorganisms to glycoproteins adsorbed to enamel or exposed root-surfaces. They may inhibit or enhance lectin mediated coaggregation between oral bacteria, and contribute to the bulk of plaque. These polysaccharides can also induce formation of antibodies that may hinder or promote development of dental caries. Although A. viscosus produces several polysaccharides their chemical fine structure remains to be elucidated. Evaluation of their role in the pathogenesis of root surface caries is also lacking, This proposal therefore aims: 1-on the surface of intact A. viscosus cells by quantitative fluorochrome-tagged lectin binding assays which yield information on the nature, number and affinity (Ka) of binding sites; 2- to isolate in purified form, cell wall, cell wall associated and extracellular polysaccharides from T6, T14V and T14Av strains of A. viscosus and characterize their molecular structure by chemical and immunochemical methods; 3- to examine the ability of isolated polysaccharides to serve as antigen inhibit attachment of bacteria to saliva coated hydroxylapatite coated beads and inhibit bacterial coaggregation; 4- to examine the antibody combining site specificity of rat and rabbit polyclonal antibody to isolate A. viscosus polysaccharide antigens; 5- to actively immunize rats with isolated polysaccharide antigens to determine the ability of specific polymers to stimulate suppression or enhancement of disease upon oral infection and 6- to prepare monoclonal rat antibodies against isolated polysaccharide antigens, elucidated their combing site specificity and evaluate the role of specific anti-carbohydrate in modifying implantation and disease in specific pathogen-free rats.