HUMAN HYBRIDOMAS IN THE ANALYSIS OF AUTOIMMUNE DISEASE

人类杂交瘤及其单抗产品将用于 在自身免疫中寻找潜在的统一特征 不同的疾病。血清交叉反应性和共享性试验 独特型将考察有限数量的克隆的可能性 参与不同的自身免疫反应。此外，假设 自身抗独特型抗体的形成在这些反应中很重要 将会受到考验。 自身免疫性血小板减少症患者的杂交瘤。K.McAdam‘s 实验室将从麻风患者身上分离出单克隆抗体 D·斯托拉的实验室将从患者身上分离出单抗 系统性红斑狼疮和相关疾病，与核和细胞交叉反应 表膜抗原。在每个实验室中，相应的抗原 将被鉴定并在核、细胞表面之间相互交叉反应 并将对细菌抗原进行测试。选定的抗体将是 部分测序，以确定是否存在复发模式 各种基因的框架和第一个高变区 自身抗体。R·施瓦茨的实验室将准备异种 抗独特型试剂，并比较上述单抗对 共有独特型。他的实验室还将测试自身抗独特型 杂交瘤培养物和血清中的抗体，并确定 它们的浓度随着疾病活动的不同而波动。

Human hybridomas and their monoclonal autoantibody products will be used to seek potentially unifying features in the autoimmunity that occurs in different diseases. Tests for serological cross-reactivity and sharing of idiotypes will examine the possibility that a restricted number of clones is involved in diverse autoimmune responses. In addition, the hypothesis that auto-anti-idiotype antibody formation is important in these responses will be tested. B. Furie's laboratory will isolate anti-platelet producing hybridomas from patients with autoimmune thrombocytopenia. K. McAdam's laboratory will isolate monoclonal antibodies from patients with leprosy with high levels of circulating immunoglobulin and antinuclear antibody. D. Stollar's laboratory will isolate monoclonal antibodies, from patients with SLE and related diseases, that cross-react with nuclear and cell surface membrane antigens. In each laboratory, the corresponding antigens will be identified and mutual cross-reactions among nuclear, cell surface and bacterial antigens will be tested. Selected antibodies will be partially sequenced to determine whether there are recurrent patterns in the framework and first hypervariable regions of the various autoantibodies. R. Schwartz's laboratory will prepare xenogeneic anti-idiotype reagents and compare the above monoclonal antibodies for shared idiotype. His laboratory will also test for auto-anti-idiotype antibodies in both hybridoma cultures and in sera, and determine whether their concentrations fluctuate in relation to disease activity.