NUTRITIONAL REGULATION OF CARCINOGENS IN PLACENTA-RELATED CELLS

多药相关的质膜糖蛋白170(P-gp) 抗性，MDR，也可以作为化学物质的外排泵 它是致癌物质，可以通过饮食和营养来调节。发现P-gp 主要分布在排列在管腔间隙的细胞中 组织，包括胎盘和妊娠子宫的子宫内膜 但P-gp在正常组织中的功能作用尚未见报道 已经确定了。我们已经确定P-gp mRNA可能是 在人类胎盘组织中受发育调节。目前，一个 大鼠发育研究正在进行中，以检测P-gp在脑内的表达 怀孕期间的正常组织。此外，几个新开发的 大鼠胎盘细胞系(由周俊英准备)正在进行测试， 体外模型。营养物质对肿瘤细胞多药耐药基因表达的调控 将研究与胎盘相关的细胞系。我们还在研究 多药耐药基因mdr1和mdr3在人宫颈病变体外模型中的表达 癌、子宫内膜腺癌和卵巢腺癌。 调节剂，如黄体酮、雌二醇和苯并(A)芘， 和营养物质，如丁酸钠、维甲酸、地塞米松和 正在检测二丁酰cAMP在表达中的调节作用 多药耐药和预防癌变。

The plasma membrane glycoprotein 170 (P-gp) responsible for multidrug resistance, MDR, also may function as a efflux pump for chemical carcinogens and can be regulated by diet and nutrients. P-gp is found predominantly in the cells lining the luminal space of a variety of tissues, including the placenta and the endometrium of the gravid uterus of the mouse, but the functional role of P-gp in normal tissues has not been determined. We have determined that P-gp mRNA may be developmentally regulated in human placental tissues. Currently, a developmental study in rats is in progress to examine P-gp expression in normal tissues during pregnancy. In addition, several newly developed rat placental cell lines (prepared by J. Y. Chou) are being tested as in vitro models. The regulation of expression of MDR by nutrients in these placenta-related cell lines will be studied. We are also examining the expression of mdr1 and mdr3 in human in vitro models, two cervical carcinomas, an endometrial adenocarcinoma, and an ovarian adenocarcinoma. Regulatory agents such as progesterone, estradiol, and benzo(a)pyrene, and nutrients such as sodium butyrate, retinoic acid, dexamethasone, and dibutyryl cAMP are being examined for regulatory roles in the expression of MDR and the prevention of carcinogenesis.