CORE--RADIOASSAY FACILITIES

核心——放射检测设施

• 批准号: 3840088
• 负责人: ARTHUR E FREEDLENDER
• 金额: --
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3840088
• 关键词: cyclic AMP; cyclic GMP; glucagon; high performance liquid chromatography; protein biosynthesis; radioimmunoassay

The Radioimmunoassay and Ligand preparation Core is a service core of the University of Virginia Diabetes Research and Training Center. It currently provides about twenty different assays or services to over forty investigators. Assays presently available include total and free serum insulin, insulin C-peptide, glucagon, cyclic AMP, and cyclic GMP radioimmunoassays; hemoglobin A, C, glucose and Lowry protein assays. In addition, (gamma 32 P) - ATP, 125 I cyclic AMP1 ligand, 125 I - peptide and proteins are produced by the core facility. We also offer and assist with HPLC techniques for separations, purifications, and analysis. Computational facilities for data from gamma counting and radioimmunoassays (tape reader, computer, printer/plotter) represent an important service of this Core. This application requests continued support for the various services provided to investigators by the Core. Critical evaluation and quality control of current services and the development of improved and/or new analytical techniques and methodologies desired by investigators (as directed by the Executive Committee) represent other benefits derived from this facility. Objectives: 1. To provide assays, reagents, radio labeled ligands, and services to DRTC Members. 2. To assist and advise on labeling, radioimmunoassay, labeled ligand preparation, and HPLC technological methods to DRTC members. 3. To assist in data calculation and evaluation. 4. To develop new analytical methods, reagents, and techniques as required by DRTC members. 5. To teach existing and new techniques to DRTC members as required.

放射免疫分析和配体制备核心是一项服务 弗吉尼亚大学糖尿病研究与培训中心 中心 它目前提供大约20种不同的测定或 为40多名调查人员提供服务。 目前可用的检测 包括总和游离血清胰岛素、胰岛素C-肽、胰高血糖素 环AMP和环GMP放射免疫测定;血红蛋白A， C，葡萄糖和Lowry蛋白测定。 此外，（γ 32 P）- ATP、125 I环AMP 1配体、125 I -肽和蛋白质。 由核心设施生产。 我们还提供并协助HPLC 分离、纯化和分析技术。 伽马计数数据的计算设施， 放射免疫测定（磁带阅读器、计算机、打印机/酶标仪） 这是核心的一项重要服务。 本申请 要求继续支持向下列机构提供的各种服务： 核心的调查员。 关键评价和质量控制 目前的服务和发展的改进和/或新的 调查人员需要的分析技术和方法 (as（由执行委员会领导） 从该设施中获得的好处。 目的： 1. 提供分析、试剂、放射性标记配体和服务 DRTC成员 2. 协助和建议标签，放射免疫测定，标记 DRTC的配体制备和HPLC技术方法 成员 3. 协助数据计算和评估。 4. 开发新的分析方法、试剂和技术 根据DRTC成员的要求。 5. 向DRTC成员教授现有技术和新技术， 必需的.