TWO-DIMENSIONAL GEL ANALYSIS OF ONCOGENE-MEDIATED TRANSFORMATION
TWO-DIMENSIONAL GEL ANALYSIS OF ONCOGENE-MEDIATED TRANSFORMATION
批准号:
3853427
负责人:
M J MILLER
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
本项目的目的是研究癌症发生的机制
使用定量二维凝胶电泳(2DG)。 这
这项技术让我们可以检查质量和数量的变化,
当细胞经历一个过程时,
肿瘤性转化 研究的重点是(1)持续
开发用于分析凝胶的计算机系统(称为ELSIE),以及(2)
使用ELSIE分析需要计算机分析的实验
的2DG。 ELSIE系统正在实验室中用于研究
不同转化癌基因对蛋白质合成的影响。
大鼠肝上皮(RLE)细胞已被分离和单细胞培养。
克隆的 这些细胞具有正常的二倍体核型,并代表了一种
均质、克隆系统转化的研究。 不同
含有转化癌基因的逆转录病毒,如v-H-ras,v-N-ras,
v-raf或v-myc/v-raf嵌合体已被用于转化RLE细胞或
人二倍体成纤维细胞系(LG-1)。 的多肽模式,
转化的细胞与其未转化的细胞显著不同,
祖先 虽然质量上的差异很少见,但超过30%的
斑点可以在数量上变化。 非常相似的癌基因,如v-H-ras
和v-N-ras,导致基本上不可区分的2DG模式。 这些
模式与由v-raf和v-myc/v-raf介导的模式不同
转型 v-raf和v-myc/v-raf模式也容易被识别。
彼此之间的区别,但更相似,而不是
未转化的或ras转化的细胞。
英文摘要
The objective of this project is to study the mechanism of carcinogenesis
using quantitative two-dimensional gel electrophoresis (2DG). This
technique lets us examine both qualitative and quantitative changes in
the synthesis of thousands of cellular polypeptides as the cell undergoes
neoplastic transformation. Research is focused on (1) the continued
development of the computer system (dubbed ELSIE) to analyze gels and (2)
the use of ELSIE to analyze experiments requiring computerized analysis
of 2DGs. The ELSIE system is being used in the laboratory to study the
effects of different transforming oncogenes on the synthesis of proteins.
Rat liver epithelial (RLE) cells have been isolated and single-cell
cloned. These cells have a normal diploid karyotype and represent a
homogeneous, clonal system for the study of transformation. Different
retrovirus containing transforming oncogenes, such as v-H-ras, v-N-ras,
v-raf, or a v-myc/v-raf chimera have been used to transform RLE cells or
a human diploid fibroblast line (LG-1). The polypeptide patterns of the
transformed cells differ significantly from their non-transformed
progenitors. While qualitative differences are rare, over 30% of the
spots may vary quantitatively. Very similar oncogenes, such as v-H-ras
and v-N-ras, cause essentially indistinguishable 2DG patterns. These
patterns are distinct from those caused by v-raf and v-myc/v-raf mediated
transformation. The v-raf and v-myc/v-raf patterns are also readily
distinguishable, but are more similar to each other than to
non-transformed or to ras transformed cells.
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专著(0)
科研奖励(0)
会议论文
GENETIC ALTERATIONS AND ALLELIC LOSS IN HUMAN HEPATOCELLULAR CARCINOMA
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批准号:6160942
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:M J MILLER
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依托单位:
A CRITICAL EVALUATION AND COMPARISON OF COMPUTERIZED SEQUENCE ANALYSIS SYSTEMS
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批准号:3774931
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:M J MILLER
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依托单位:
PLASMA PROTEINS AS EARLY BIOMARKERS OF EXPOSURE TO CARCINOGENIC AROMATIC AMINES
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批准号:3853569
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:M J MILLER
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依托单位:
DEVELOPMENT OF AN RLE CELL CDNA LIBRARY FOR CELL-FREE EXPRESSION OF PROTEINS
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批准号:3838484
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:M J MILLER
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依托单位:
COMPUTER ANALYSIS OF CARCINOGENESIS BY TWO-DIMENSIONAL GEL ELECTROPHORESIS
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批准号:3896355
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:M J MILLER
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依托单位:
TWO-DIMENSIONAL GEL ANALYSIS OF CARCINOGENESIS
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批准号:3774790
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:M J MILLER
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依托单位:
TWO-DIMENSIONAL GEL ANALYSIS OF CARCINOGENESIS
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批准号:3838340
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:M J MILLER
-
依托单位:
COMPUTER ANALYSIS OF CARCINOGENESIS BY TWO-DIMENSIONAL GEL ELECTROPHORESIS
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批准号:4692350
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:M J MILLER
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依托单位:
COMPUTER ANALYSIS OF CARCINOGENESIS BY TWO-DIMENSIONAL GEL ELECTROPHORESIS
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批准号:3916774
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:M J MILLER
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依托单位:
GENETIC ALTERATIONS AND ALLELIC LOSS IN HUMAN HEPATOCELLULAR CARCINOMA
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批准号:2463664
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M J MILLER
-
依托单位:
COMPUTER ANALYSIS OF CARCINOGENESIS BY TWO-DIMENSIONAL GEL ELECTROPHORESIS
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批准号:3963451
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:M J MILLER
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依托单位:
海外基金