BIOMEDICAL RESEARCH SUPPORT

生物医学研究支持

• 批准号: 3516079
• 负责人: MATTHEW NATHAN LEVY
• 金额: $ 0.8万
• 依托单位: MOUNT SINAI MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-04-01 至 1992-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3516079
• 关键词: health science research support; hospitals

This proposal requests funds for the purchase of optical and electronic equipment to perform dual-wavelength fluorescence measurements in isolated single cells, small multicellular preparations and crystalline enzymes. The equipment will be shared by six Project Leaders (all with active NIH grants) in three departments at the University of California, Riverside. The projects are studies involved in general areas of cardiovascular disease, cystic fibrosis, diabetes, endocrinology of Ca homeostasis (e.g. Vitamin D & PTH), toxicology, neuromuscular transmission and molecular aspects of enzyme catalysis. The following projects are described. Drs. Bers & Briggs will study the regulation of cytoplasmic Ca (and Nai & pHi) and Ca-currents in isolated cardiac myocytes, with respect to the sources of Ca which support contraction (SR & Ca influx) and also localization of arrhythmogenic sites. Dr. Luben will study the regulation of cytoplasmic Ca in suspensions of bone cells treated with parathyroid hormone (PTH) and in single bone cells (to study subcellular Ca distribution) and in cells transfected with the PTH receptor. Drs. Norman and Nemere will determine the subcellular localization of Cai (using fura-2) and beta-tubulin (using fluorescent antibodies) in isolated intestinal epithelial cells as functions of vitamin D status. Dr. Quinton will measure changes in [Ca]i, pHi and electrophysiological parameters in response to isotonic absorptive loads in isolated sweat gland ducts from both normal and cystic fibrosis subjects. Dr. Adams will use fluorescent Ca indicators to study the mechanism by which a novel spider toxin, omega-agatoxin inhibits Ca influx in presynaptic nerve terminals at neuromuscular junctions. He will also examine the means by which the neuropeptide proctolin mediates Ca entry in skeletal muscle. Dr. Dunn will study molecular interactions between enzymes and cofactors during different steps in the reaction sequence of four enzymes in structured (crystalline) states. The changes in polarization and spectral properties of intrinsically fluorescence cofactors will be measured.

本提案要求提供资金，用于购买光学和电子设备， 设备进行双波长荧光测量在隔离 单细胞、小的多细胞制剂和晶体酶。 该设备将由六名项目负责人（均与NIH合作）共享 在滨江的加州大学的三个系中， 这些项目是涉及心血管一般领域的研究 疾病、囊性纤维化、糖尿病、Ca稳态的内分泌学（例如， 维生素D和甲状旁腺素），毒理学，神经肌肉传递和分子 酶催化的一些方面。 对以下项目进行了说明。 Drs. Bers & Briggs将研究细胞质Ca（和Nai & pHi）和钙电流的影响。 支持收缩（SR和Ca流入）的Ca来源， 致突变位点的定位。 博士Luben将研究悬浮液中细胞质Ca的调节， 用甲状旁腺激素（PTH）处理的骨细胞和单个骨细胞 (to研究亚细胞Ca分布）和用 甲状旁腺素受体 Drs. Norman和Nemere将确定Cai的亚细胞定位 （使用fura-2）和β-微管蛋白（使用荧光抗体）在分离的 肠上皮细胞作为维生素D状态的功能。 博士Quinton将测量[Ca]i，pHi和电生理学的变化。 在离体汗腺中响应于等渗吸收负荷的参数 来自正常和囊性纤维化受试者的导管。 博士亚当斯将使用荧光钙指示剂，通过 一种新的蜘蛛毒素，欧米茄-agatoxin抑制钙内流， 神经肌肉接头处的突触前神经末梢。 他还将 检查神经肽proctolin介导Ca进入的方式， 骨骼肌 博士邓恩将研究酶和辅因子之间的分子相互作用 在四种酶的反应顺序的不同步骤中， 晶体（crystalline）状态。 偏振和光谱的变化 将测量固有荧光辅因子的性质。