BIOMEDICAL RESEARCH SUPPORT

生物医学研究支持

• 批准号: 3516723
• 负责人: JUAN M. NAVIA
• 金额: $ 1.19万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1992-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3516723
• 关键词: health /scientific organization; health science research support

We are requesting funds to purchase the ACAS 570 Interactive Laser Cytometer which is marked by Meridian Instruments of Okemos, MI. This instrument is an integrated fluorescence workstation which is designed for quantitative fluorescence analysis of adherent cells in culture and for interactive applications, such as fluorescence photobleaching to measure diffusion of molecules on or within cells as well s between cells, laser ablation for sorting and selection of anchored cells, and cell surgery for selective removal of organelles or cell processes. The projects described in this Shared Instrumentation Grant will employ various applications of the ACAS protein mobility of cell surfaces, cell-cell communication studies, intracellular Ca2+ and pH measurements of anchorage-dependent cells as well as immunofluorescence staining to obtain both spatial and temporal resolution of various cellular processes and the distribution of specific antigens in neurons, astrocytes, tumor cells, and oncogene- transformed cells. In comparison to flow cytofluorimetric methods and fluorescence-activated cell sorting (FACS), the ACAS system offers three distinct advantages which are necessary for the studies proposed: 1) the ability to analyze and sort anchored cells without having to strip them from their substratum or connecting cells (and possibly causing irreversible and inestimable damage to cellular structure and function); 2) the markers over a prolonged period of time; and 3) the ability to interact with the cells under study (eg., the fluorescence photobleaching applications). Thus, rather than an alternative to the more conventional instruments, the ACAS 570 Interactive Laser Cytometer is complementary to them.

我们正在申请购买ACAS 570交互式激光器的资金 由密歇根州奥克莫斯的子午线仪器公司标记的细胞仪。这 仪器是一个集成的荧光工作站，专为 贴壁细胞在培养和培养过程中的荧光定量分析 交互式应用，如荧光光漂白测量 分子在细胞上或细胞内的扩散以及细胞间的扩散，S，激光 用于分离和选择锚定细胞的消融，以及用于 选择性去除细胞器或细胞突起。所描述的项目 在此共享仪器拨款中，将采用各种应用程序 ACAS蛋白在细胞表面的迁移率、细胞间的通讯 锚定依赖的细胞内钙离子和pH的测定 细胞以及免疫荧光染色，以获得空间和 不同细胞过程的时间分辨率和分布 神经元、星形胶质细胞、肿瘤细胞和癌基因中的特定抗原- 转化的细胞。与流式细胞荧光法和 荧光激活细胞分选(FACS)，ACAS系统提供三种 建议的研究所需的独特优势：1) 无需剥离锚定单元格即可对其进行分析和排序 从它们的底物或连接的细胞(并且可能导致 对细胞结构和功能造成不可逆转和不可估量的损害)；2) 长时间的标记；以及3)交互的能力 利用研究中的细胞(例如，荧光光漂白 应用程序)。因此，它不是更传统的选择 ACAS 570交互式激光细胞仪是对以下仪器的补充 他们。