TRIPLE QUADRUPOLE ELECTROSPRAY MASS SPECTROMETER

三重四极杆电喷雾质谱仪

• 批准号: 3520937
• 负责人: SIMON J GASKELL
• 金额: $ 32.9万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-12 至 1992-08-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520937
• 关键词: analytical chemistry; biomedical equipment purchase; ionization; mass spectrometry; peptides

This Shared Instrumentation Grant application is for the purchase of an electrospray triple quadrupole mass spectrometer for the qualitative and quantitative analysis of peptides and other compounds of biomedical importance. The requested amount is based on the purchase of a VG Instruments Bio-Q instrument which presently represents the most economical system available with the required performance. It would be prudent, however, should this application be successful, to assess currently available instrumentation for electrospray triple quadrupole mass spectrometry at the time of funding. The proposed purchase would provide a significant extension to the mass spectrometry instrumentation (principally a hybrid tandem instrument equipped for fast atom bombardment ionization) presently employed and heavily committed in a number of research areas. Thus, the new instrument would (i) allow the establishment of additional collaborative programs not presently feasible because of pressure on a single instrument, and (ii) permit new applications of mass spectrometry by exploiting the unique properties of the recently devised electrospray procedure. Preliminary data obtained by the Program Director in collaboration with an existing user and one of the instrument manufacturers have established that superior sensitivities of detection may be achieved in the analyses of certain peptides (such as the vasoactive peptide, endothelin) using electrospray, thus establishing the feasibility of their trace analysis in biological extracts. In addition, the ability of the electrospray technique to achieve ionization of relatively large peptides opens new areas of application of mass spectrometry, for example in the characterization of xenobiotic/peptide conjugates. Furthermore, the typical generation of multiply charged ions by electrospray extends the mass range of analytes for which useful structural information may be obtained following collisional activation during tandem MS. Investigators who will share the new instrumentation and subjects of their research are as follows: K. Angelides (location and kinetics of peptide phosphorylation); R.G. Cook (confirmation of structure of synthetic peptides); S.J. Gaskell (characterization and quantification of peptide adducts, including leukotrienes and xenobiotic conjugates); H.F. Gilbert (characterization of peptide disulfide bridges); J. Moake/M. Phillips (characterization of polymeric aurin tricarboxylid acid, an inhibitor of von Willebrand Factor- mediated platelet aggregation); P.M. Vanhoutte (quantification of endothelin); C.-Y. Yang (characterization of modified apolipoprotein-B). Additionally and importantly, the Program Director is committed to a program of research to improve understanding of the analytical principles involved in these applications (such as the factors influencing the fragmentation of multiply charged ions).

此共享仪器补助金申请用于购买 电喷雾三重四极杆质谱仪用于定性和 肽和其他生物医学化合物的定量分析 重要性。 请求的金额基于购买 VG 仪器 Bio-Q 仪器是目前最经济的仪器 系统具有所需的性能。 应该谨慎起见， 然而，如果该申请成功，评估目前 用于电喷雾三重四极杆质量的可用仪器 资助时的光谱测定。 拟议的购买将提供 质谱仪器的重大扩展 （主要是配备用于快速原子轰击的混合串联仪器 电离）目前在许多领域得到广泛应用和大力投入 研究领域。 因此，新文书将 (i) 允许建立 额外的合作计划目前不可行，因为 对单个仪器施加压力，并且（ii）允许质量的新应用 通过利用最近设计的独特性能进行光谱测定 电喷雾程序。 项目主任获得的初步数据 与现有用户和其中一台仪器合作 制造商已经确定，卓越的检测灵敏度可能 可以在某些肽（例如血管活性肽）的分析中实现 肽，内皮素）使用电喷雾，从而建立了可行性 生物提取物中的痕量分析。 此外，能力 电喷雾技术实现相对较大的电离 例如，肽开辟了质谱分析的新应用领域 异生素/肽缀合物的表征。 此外， 通过电喷雾典型地产生多电荷离子，延长了 可以提供有用结构信息的分析物的质量范围 在串联 MS 期间碰撞激活后获得。 调查员 谁将分享新仪器和他们的研究主题 如下：K. Angelides（肽磷酸化的位置和动力学）； R.G. Cook（合成肽结构的确认）； S.J.加斯凯尔 （肽加合物的表征和定量，包括 白三烯和异生素结合物）； H.F.吉尔伯特（表征 肽二硫桥）； J.莫克/M。菲利普斯（表征 聚合金精三羧基酸，血管性血友病因子抑制剂- 介导的血小板聚集）；下午。 Vanhoutte（量化 内皮素）； C.-Y。 Yang（修饰载脂蛋白-B 的表征）。 此外且重要的是，项目总监致力于 提高对分析原理的理解的研究计划 涉及到这些应用（例如影响因素 多电荷离子的碎裂）。