TRIPLE QUADRUPOLE ELECTROSPRAY MASS SPECTROMETER

此共享仪器授权应用程序用于购买 电喷雾三重四极杆质谱仪用于定性和定量 生物医学中的多肽等化合物的定量分析 重要性。申请的金额是基于购买一辆VG Bio-Q仪器是目前最经济的仪器 提供具有所需性能的系统。这样做是谨慎的， 但是，如果这个申请成功了，目前要评估 可用于电喷雾三重四极杆质量的仪器 在提供资金的时候，我们可以使用光谱分析技术。拟议的收购将提供 对质谱仪的重大扩展 (主要是配备了用于快速原子轰击的混合串列仪器 电离)目前在许多方面使用并严重致力于 研究领域。因此，新文书将(I)允许建立 由于以下原因，其他协作计划目前尚不可行 单个仪器上的压力，以及(Ii)允许新的质量应用 利用最近设计的光谱分析仪器的独特性能 电喷雾程序。项目主任获得的初步数据 与现有用户和其中一个仪器协作 制造商已经证实，超高的检测灵敏度可以 在某些多肽(如血管活性物质)的分析中实现 肽、内皮素)使用电喷雾，从而确立了可行性 它们在生物提取物中的痕量分析。另外，这个能力， 的电喷雾技术实现了较大的电离 例如，多肽开辟了质谱学应用的新领域 在异源/多肽结合物的表征中。此外， 通过电喷雾产生的典型多电荷离子扩展了 可能具有有用结构信息的分析物的质量范围 在串联过程中碰撞激活后获得的信息 谁将分享新的仪器和他们研究的主题是 K.Angelide(多肽磷酸化的定位和动力学)； R.G.库克(合成肽结构的确认)；S.J.Gaskell (多肽加合物的表征和定量，包括 白三烯和异物结合物)；H.F.吉尔伯特(表征 多肽二硫键)；J.Moake/M.Phillips(表征 Von Willebrand因子的抑制剂--聚合金三羧酸-- 调节的血小板聚集)；PM Vanhoutte(量化 内皮素)；杨振元(修饰载脂蛋白-B的特性)。 此外，也是重要的是，计划主任致力于 提高对分析原理的理解的研究方案 涉及这些应用程序(例如影响 多电荷离子的碎裂)。

This Shared Instrumentation Grant application is for the purchase of an electrospray triple quadrupole mass spectrometer for the qualitative and quantitative analysis of peptides and other compounds of biomedical importance. The requested amount is based on the purchase of a VG Instruments Bio-Q instrument which presently represents the most economical system available with the required performance. It would be prudent, however, should this application be successful, to assess currently available instrumentation for electrospray triple quadrupole mass spectrometry at the time of funding. The proposed purchase would provide a significant extension to the mass spectrometry instrumentation (principally a hybrid tandem instrument equipped for fast atom bombardment ionization) presently employed and heavily committed in a number of research areas. Thus, the new instrument would (i) allow the establishment of additional collaborative programs not presently feasible because of pressure on a single instrument, and (ii) permit new applications of mass spectrometry by exploiting the unique properties of the recently devised electrospray procedure. Preliminary data obtained by the Program Director in collaboration with an existing user and one of the instrument manufacturers have established that superior sensitivities of detection may be achieved in the analyses of certain peptides (such as the vasoactive peptide, endothelin) using electrospray, thus establishing the feasibility of their trace analysis in biological extracts. In addition, the ability of the electrospray technique to achieve ionization of relatively large peptides opens new areas of application of mass spectrometry, for example in the characterization of xenobiotic/peptide conjugates. Furthermore, the typical generation of multiply charged ions by electrospray extends the mass range of analytes for which useful structural information may be obtained following collisional activation during tandem MS. Investigators who will share the new instrumentation and subjects of their research are as follows: K. Angelides (location and kinetics of peptide phosphorylation); R.G. Cook (confirmation of structure of synthetic peptides); S.J. Gaskell (characterization and quantification of peptide adducts, including leukotrienes and xenobiotic conjugates); H.F. Gilbert (characterization of peptide disulfide bridges); J. Moake/M. Phillips (characterization of polymeric aurin tricarboxylid acid, an inhibitor of von Willebrand Factor- mediated platelet aggregation); P.M. Vanhoutte (quantification of endothelin); C.-Y. Yang (characterization of modified apolipoprotein-B). Additionally and importantly, the Program Director is committed to a program of research to improve understanding of the analytical principles involved in these applications (such as the factors influencing the fragmentation of multiply charged ions).