MOLECULAR BIOLOGY CORE INSTRUMENTATION FACILITY

将建立一个仪器仪表核心， 在分子生物学和分子遗传学方面正在进行的各种项目 正在进行的大多数实验室在该部 生物科学。 要求提供下列新设备： 计算机化的二维密度计， 放射性扫描仪，DNA测序仪，- DNA/RNA提取器和 自动移液器/结晶器。 该设施还将包括DNA 合成器、荧光计、HPLC和高压纸 电泳站 该设施将受到监督和维护 由一名专门的技术人员，谁也将进行DNA合成， DNA测序凝胶运行。 该设施的主要用户正在研究 二氢叶酸还原酶基因在培养哺乳动物中的表达 细胞，增强子在免疫球蛋白基因调控中的作用， RNA剪接和31末端形成机制，DNA肿瘤病毒 复制，c-fos癌基因的转录调控， 酵母中线粒体的生物发生、转录终止和 抗终止E.大肠杆菌，控制基因的表征 C.线虫的分子遗传学 果蝇的形态发生 该设施将提供给所有 生物系24个实验室的研究人员 在哥伦比亚晨边校园的科学。 几个这样 与人类疾病直接相关的项目（e. G.，癌基因， 肿瘤病毒，环境诱变剂，神经变性），但对于 它们大部分代表遗传学、生物化学， 发展和神经生物学。

A core of instrumentation will be established to facilitate and promote a variety o ongoing projects in molecular biology and molecular genetics being carried out in moste of the laboratories in the Department of Biological Sciences. The following new equipment items are requested: a computerized 2-dimensional densitometer, - a 2dimensional radioactivity scanner, a DNA sequencer, a - DNA/RNA extractor, and an automated pipettor/crystallizer. The facility will also include a DNA synthesizer, a fluorimeter, an HPLC, and a high voltage paper electrophoresis station. The facility will be supervised and maintained by a dedicated technician, who will also carry out the DNA syntheses and the DNA sequencing gel runs. Major users of this facility are studying the expression of the dihydrofolate reductase gene in cultured mammalian cells, the role of the enhancer in immunoglobulin gene regulation, the mechanism of RNA splicing and 31 end formation, DNA tumor virus replication, transcriptional regulation of the c-fos oncogene, the biogenesis of mitochondria in yeast, transcription termination and antitermination in E. coli, the characterization of genes controlling cell differentiation in C. elegans, and the molecular genetics of morphogenesis in Drosophila. The facility will be available to all researchers in the 24 laboratories of the Department of Biological Sciences at the Morningside Campus at Columbia. Several of these projects have direct relevance to human disease (e. g. , oncogenes, tumor viruses, environmental mutagens, nerve degeneration) but for the most part they represent basic research in genetics, biochemistry, development, and neurobiology.