ELECTRON MICROSCOPE AND MICRODENSITOMETER

电子显微镜和显微密度计

• 批准号: 3521012
• 负责人: Paula F. Flicker
• 金额: $ 28.8万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-10 至 1992-05-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521012
• 关键词: biomedical equipment purchase; cryoscopy; electron microscopy

It is proposed that a Philips CM12 transmission electron microscope and an Optronics C-4100SP microdensitometer be purchased. These instruments are necessary for structural studies by members of the research groups of Drs. Flicker, Fleischer, Robinson, Stubbs, LeStourgeon, and Williams. Dr. Flicker is examining the structure of actin and myosin. She plans to utilize cryo-electron microscopy to visualize different conformational states of actomyosin and localize functionally significant sites on the proteins. Dr. Robinson has developed a procedure for preparing two-dimensional crystals of neurotoxins, well suited for examination using electron microscopic techniques. Dr. Fleischer works on channels involved in excitation-contraction coupling in muscle. His goal is to examine the structures of the channels in open and closed states using cryo-electron microscopy. To date, Drs. Robinson and Fleischer have had to depend on external collaborations to obtain data for electron microscopy and image processing. Dr. Stubbs is determining molecular structure of helical plant viruses, Dr. LeStourgeon is concerned with the structure of nuclear ribonucleoprotein particles, and Dr. Williams studies microtubules and microtubule-associated proteins. Studies of all of these protein and nucleic acid assemblies would benefit from the relatively high resolution and state of preservation available through cryo-electron microscopy.

建议Philips CM 12透射电镜 和Optronics C-4100 SP显微密度计。 这些 仪器是必要的结构研究的成员， 弗里克博士、弗莱舍博士、罗宾逊博士、斯塔布斯博士 LeStourgeon和威廉姆斯。 弗里克博士正在研究肌动蛋白和肌球蛋白的结构。 她 计划利用低温电子显微镜观察不同的 肌动球蛋白的构象状态和定位功能意义 蛋白质上的位点。 罗宾逊医生发明了一种方法 制备神经毒素的二维晶体， 用电子显微镜技术检查。 弗莱舍博士 肌肉中参与兴奋-收缩耦合的通道。 他 目的是检查开放和封闭通道的结构 状态使用cryo-electron显微镜。 迄今为止，罗宾逊博士和 弗莱舍不得不依靠外部合作来获取数据 用于电子显微镜和图像处理。 斯塔布斯博士正在确定螺旋植物的分子结构 LeStourgeon博士关注的是核结构， 核糖核蛋白颗粒，威廉姆斯博士研究微管和 微管相关蛋白。 对所有这些蛋白质和 核酸组装将受益于相对较高的分辨率 和保存状态。

项目成果

Insulinoma: diagnosis and management.

胰岛素瘤：诊断和治疗。

• 发表时间: 1980
• 期刊: Boletin de la Asociacion Medica de Puerto Rico
• 作者: AguiloJr,F;Rabell,VM;Allende,M;VazquezQuintana,E
• 通讯作者: VazquezQuintana,E