CO DEHYDROGENASE AND ACETOCLASTIC METHANOGENESIS IN METHANOSARCINA BARKERI

巴克甲烷八球菌中的 CO 脱氢酶和乙酰分解产甲烷作用

• 批准号: 3878878
• 负责人: D A GRAHAME
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3878878
• 关键词: Methanobacteriaceae; acetates; adenosine triphosphate; anaerobic bacteria; bioenergetics; carbon monoxide; enzyme mechanism; enzyme structure; hydroxymethyltransferases; isozymes; methane; methyl group; methyltransferase; microorganism culture; microorganism metabolism; tissue /cell culture; transmethylation

Acetic acid is a major metabolic product of bacteria which anaerobically decompose complex organic materials. In certain natural microbial ecosystems and in those used by man for waste treatment the strictly anaerobic microorganism Methanosarcina barkeri is of prime importance in subsequent conversion of acetic acid to methane and carbon dioxide. The enzymes involved in acetic acid metabolism have been studied in this project since detailed information about this process is lacking. Steps involved in cleavage of the carbon-carbon bond of acetate have been studied by analysis of the enzyme carbon monoxide dehydrogenase (CODH). Subsequent methyl group transfer steps are involved prior to methane formation and previously this project has identified, purified, and produced antibodies against two methyl group transferase enzymes. One of these is present largely in cells grown on methanol, and the other is predominant in cells capable of acetate conversion to methane and carbon dioxide. Studies on these enzymes have been extended in order to compare their structural and catalytic properties in detail.

乙酸是细菌厌氧代谢的主要产物， 分解复杂的有机物质在某些天然微生物中 生态系统和人类用于废物处理的生态系统中， 厌氧微生物巴氏甲烷八叠球菌在 随后将乙酸转化为甲烷和二氧化碳。的 参与乙酸代谢的酶已经在这方面进行了研究。 由于缺乏有关这一过程的详细信息，步骤 参与裂解的碳-碳键的乙酸酯已被研究 通过一氧化碳脱氢酶（CODH）的分析。后续 在甲烷形成之前涉及甲基转移步骤， 在此之前，该项目已经鉴定、纯化和生产了抗体， 针对两种甲基转移酶。其中之一是目前 大部分在甲醇上生长的细胞中，另一种在细胞中占主导地位 能够将乙酸盐转化为甲烷和二氧化碳。研究 这些酶已经被扩展以比较它们的结构和 催化剂的特性。