DNA SEQUENCE CORE FACILITY

DNA 序列核心设施

• 批准号: 3520573
• 负责人: MARK A MCINTOSH
• 金额: $ 15.2万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-11 至 1991-05-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520573
• 关键词: DNA replication; automated data processing; biomedical equipment purchase; biomedical equipment resource; computer assisted sequence analysis; proteins

The objective of this application is to obtain the necessary state-of-the- art instrumentation for expansion of the existing University of Missouri DNA Core Facility to provide services in automated nucleotide sequencing and immediate information access to sequence analysis software and DNA/protein databases. To achieve this objective, we are requesting capital funds to purchase an integrated instrumentation/hardware/software system consisting of (i) an automated DNA sequencing apparatus (ABI 370A), (ii) a dedicated oligonucleotide synthesizer (ABI 380B) to generate sequencing primers for this service, and (iii) a computer network (Sun Microsystems, Inc.) linking at least 4 basic science departments and greater than 50 present laboratories (with room for expansion) to a central facility for on-site access to the Intelligenetics Suite of software programs and the three major DNA and protein databases. This proposal describes PHS-supported research activities of investigators who currently generate extensive DNA sequence data and require immediate analysis capabilities (major users) and other investigators whose sequence requirements are limited at present, but with the likely possibility of future expansion (minor users). The acquisition of this equipment will greatly increase the speed and accuracy with which these data can be generated and analyzed for both user groups. It is anticipated that these user groups will represent 80-90% of the total usage of the requested equipment; the remaining time will be utilized for training and workshops aimed at developing such research capabilities in other research programs on campus. The University of Missouri Molecular Biology Program has committed resources for the maintenance and operation of this expansion of the existing DNA core facility.

本申请的目的是获得必要的最新 现有密苏里大学扩展的艺术仪器 DNA核心设备可在自动核苷酸测序中提供服务 并立即信息访问序列分析软件和 DNA/蛋白质数据库。 为了实现这一目标，我们要求 购买集成仪器/硬件/软件的资本资金 由（i）自动DNA测序设备（ABI 370A）组成的系统， （ii）专用寡核苷酸合成器（ABI 380B）生成 为此服务进行测序引物，以及（iii）计算机网络（太阳 Microsystems，Inc。）至少连接4个基础科学部门和 大于50个现在的实验室（有扩展的空间）到中央 用于现场访问软件智能套件的设施 程序以及三个主要的DNA和蛋白质数据库。 这个建议 描述了目前的研究人员的PHS支持研究活动 生成广泛的DNA序列数据，需要立即分析 功能（主要用户）和其他调查人员的序列 目前要求有限，但可能有可能 未来扩展（次要用户）。 收购此设备将 大大提高了这些数据的速度和准确性 对两个用户组生成和分析。 预计这些 用户组将代表所请求的总用途的80-90％ 设备;剩余时间将用于培训和研讨会 旨在在其他研究计划中发展此类研究能力 在校园里。 密苏里大学分子生物学计划已有 为维护和运营的授权资源 现有的DNA核心设施。