RAPID X-RAY DATA COLLECTION SYSTEM

快速 X 射线数据采集系统

• 批准号: 3520396
• 负责人: F SCOTT MATHEWS
• 金额: $ 16.5万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-24 至 1990-05-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520396
• 关键词: X ray crystallography; biomedical equipment purchase; data collection; information systems; protein structure function

Funds for a modern x-ray data collection facility based on electronic area detector instrumentation are requested in this proposal. Rapid changes in DNA technology have led to a growing interest in the structural basis of protein function. Consequently an increasing number of investigators are turning to x-ray crystallography to obtain three-dimensional structures. At Washington University School of Medicine, the growing interest in conformational analysis is hindered by a serious deficiency in instrumentation for x-ray data collection. The only available equipment exists in the laboratories of two crystallographic groups, one in the Department of Biological Chemistry and the other in the Department of Cell Biology and Physiology. This equipment is considerably outdated and scientists in the crystallography groups have already found it necessary to collect diffraction data at resources outside of St. Louis. Continued progress on current and rapidly evolving structural projects within the crystallography groups and throughout the School of Medicine, many relying heavily on recombinant DNA technology, is possible only if this equipment becomes available. By using area detectors to record the x-ray diffraction data, at least a fifty-fold increase in the data collection rate could be obtained. In addition, further advantages would include obtaining of better data, the ability to utilize smaller or radiation sensitive protein crystals, and improved phasing through recordings of anomalous scattering information not readily obtained from large unit cells by diffractometers. The request which follows describes the existing equipment, research projects which would benefit greatly from the instrumentation, and ongoing efforts to train protein chemists in the use of diffraction methods. Most of the projects employ site- directed mutagenesis and/or the cloning of proteins and will be nearly impossible to pursue without the requested instrumentation.

为建立一个现代化的X射线数据收集设施提供资金 在此需要电子区域探测器仪器 求婚。 DNA技术的快速变化导致了人们对 蛋白质功能的结构基础。因此， 越来越多的调查人员转向x光检查 结晶学来获得三维结构。在… 华盛顿大学医学院，越来越多的人对 构象分析受到一个严重缺陷的阻碍。 用于X射线数据收集的仪器。唯一可用的 在两个结晶学实验室里有设备 小组，一个在生物化学系，另一个在生物化学系 在细胞生物学和生理学系。这台设备 已经相当过时了，结晶学中的科学家们 研究小组已经发现有必要收集衍射数据 在圣路易斯以外的资源中心。在当前的基础上继续取得进展 以及结晶学中快速发展的结构项目 团体和整个医学院，许多人严重依赖 在重组DNA技术上，只有当这个设备 变得可用。 通过使用面积探测器记录x射线衍射数据，在 数据采集率至少提高50倍可能是 获得。此外，进一步的优势将包括获得 更好的数据，利用更小或更辐射的能力 敏感的蛋白质晶体，并通过录音改进了相变 反常散射信息不容易从大的 用衍射仪对细胞进行单元测量。 下面的请求描述了现有设备， 将大大受益于 仪器，以及持续努力培训蛋白质化学家 衍射法的使用。大多数项目都雇用了工地- 定向突变和/或蛋白质克隆，并将被 如果没有所要求的仪器，几乎不可能继续下去。