STUDIES OF PHOTOTHERAPY FOR THORACIC MALIGNANCIES

胸部恶性肿瘤光疗的研究

• 批准号: 3939564
• 负责人: H I PASS
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939564
• 关键词: cytotoxicity; disease /disorder model; human subject; light scattering; lung neoplasms; metastasis; neoplasm /cancer photoradiation therapy; porphyrins; radiosensitizer; tissue /cell culture; trachea neoplasm

Our laboratory has investigated the use of photodynamic therapy (PDT) for the treatment of thoracic malignancies by sensitization of malignant cells with a porphyrinoid (HPD) followed by illumination with 630 nM light. The in vitro sensitivity of human lung cancer line A 549 has been studied revealing that the cytotoxicity is dependent on the amount of energy input as well as the sentizer concentration used. A dose-rate effect has been revealed when cells were treated with .09 mW/cm2 vs. .27 mW/cm2. Cells incubated longer with the sensitizer had a significantly less surviving fraction by colony formation assay. Differences in in vitro sensitivity correlated with degree of cellular HPD concentration as measured by fluorescence, the protein concentration of the cells, as well as the size of the cells. Use of a light scattering media (intralipid) was able to augment the PDT cytotoxicity despite decreases in cellular HPD. Animal models of pulmonary metastases, tracheal tumors, as well as subcutaneous models have been established using the F344 rat sarcoma for the testing in vivo PDT. In vitro PDT of the F 344 rat sarcoma cell line has revealed it also to be sensitive to PDT. Two patients with complete bronchial obstruction due to metastatic malignancies have been palliated with PDT with partial or total lung expansion in both cases, without morbidity.

我们的实验室已经研究了光动力疗法的使用 (PDT)敏化治疗胸部恶性肿瘤 恶性细胞与类卟啉(Hpd)的关系 用630纳米的灯光照明。人的体外敏感性 对肺癌A549株的研究表明， 细胞毒性取决于能量输入的量以及 感情用事所用的浓度。剂量率效应已经被 当细胞以0.09 mW/cm2与0.27 mW/cm2处理时显示 MW/cm2。与增敏剂孵育时间较长的细胞具有 集落形成实验显示存活率显著降低。 体外敏感性的差异与其程度有关 用荧光法测量细胞内HpD浓度， 细胞的蛋白质浓度以及细胞的大小。 使用光散射介质(Intralipid)能够增加 尽管细胞内HPD减少，但PDT的细胞毒性。动物 肺转移模型、气管肿瘤模型以及 用F344大鼠建立了皮下模型 对肉瘤进行体内PDT检测。F344细胞的体外光动力疗法 大鼠肉瘤细胞株也显示对PDT敏感。 支气管镜检查致完全性支气管阻塞2例 转移性恶性肿瘤已通过PDT和 两例均为部分或全部肺扩张，无并发症。