STUDIES OF PHOTOTHERAPY FOR THORACIC MALIGNANCIES

我们的实验室研究了光动力疗法的应用 (PDT)用于通过致敏治疗胸部恶性肿瘤 恶性细胞与卟啉（HPD），然后 用630 nM光照射。 人的体外敏感性 肺癌细胞系A549的研究表明， 细胞毒性取决于能量输入的量， 使用的浓度。 剂量率效应已经被 当细胞用0.09 mW/cm 2与0.27 mW/cm 2 mW/cm 2。与致敏剂一起孵育更长时间的细胞具有 通过集落形成测定，存活分数显著更低。 体外敏感性的差异与 通过荧光测量的细胞HPD浓度， 细胞的蛋白质浓度以及细胞的大小。 使用光散射介质（intraperoid）能够增加 PDT的细胞毒性，尽管细胞HPD减少。 动物 肺转移瘤、气管肿瘤以及 用F344大鼠建立皮下模型 用于体内PDT测试。 F344的体外PDT 大鼠肉瘤细胞系也显示其对PDT敏感。 2例完全性支气管阻塞， 转移性恶性肿瘤已经用PDT缓解， 两种情况下肺部分或全部扩张，无发病率。

Our laboratory has investigated the use of photodynamic therapy (PDT) for the treatment of thoracic malignancies by sensitization of malignant cells with a porphyrinoid (HPD) followed by illumination with 630 nM light. The in vitro sensitivity of human lung cancer line A 549 has been studied revealing that the cytotoxicity is dependent on the amount of energy input as well as the sentizer concentration used. A dose-rate effect has been revealed when cells were treated with .09 mW/cm2 vs. .27 mW/cm2. Cells incubated longer with the sensitizer had a significantly less surviving fraction by colony formation assay. Differences in in vitro sensitivity correlated with degree of cellular HPD concentration as measured by fluorescence, the protein concentration of the cells, as well as the size of the cells. Use of a light scattering media (intralipid) was able to augment the PDT cytotoxicity despite decreases in cellular HPD. Animal models of pulmonary metastases, tracheal tumors, as well as subcutaneous models have been established using the F344 rat sarcoma for the testing in vivo PDT. In vitro PDT of the F 344 rat sarcoma cell line has revealed it also to be sensitive to PDT. Two patients with complete bronchial obstruction due to metastatic malignancies have been palliated with PDT with partial or total lung expansion in both cases, without morbidity.