AMINOACYL-TRNAS IN HIV AND OTHER RETROVIRAL INFECTED CELLS

转移RNA分离自HIV-I（人类免疫缺陷病毒-HIV-I）。 1、HIV-2（人类免疫缺陷病毒-2）、HTLV-I（人类T细胞 白血病病毒-1），HIV-病毒）和BLV，（牛白血病病毒）- 感染的和未感染的细胞以及 比较了感染和未感染细胞的氨酰-tRNA 通过反相色谱法。 在每一种情况下检查，Asn-tRNA， 其通常在5 '端含有高度修饰的Q碱基， 其反密码子的位置，在感染的 细胞 此外，来自HIV-1和HIV-2感染细胞的Phe-tRNA 缺乏高度修改的怀伊基地在3 '侧， 反密码子 有趣的是，这些tRNA中的一种或两种都发生在或 在gag-pol表达的核糖体移码位点附近 每个逆转录病毒的多聚蛋白。 可能发挥的作用 核糖体移码中低修饰碱基目前正在 研究了

Transfer RNA was isolated from HIV-I (human immunodeficiency virus- 1, HIV-2 (human immunodeficiency virus-2), HTLV-I (human T-cell leukemia virus-1), HIV- virus) and BLV, (bovine leukemia virus)- infected and -uninfected cells and the elution profiles of aminoacyl-tRNAs from infected and uninfected cells were compared by reverse phase chromatography. In each case examined, Asn-tRNA, which normally contains the highly modified Q base in the 5' position of its anticodon, was deficient in this base in infected cells. Phe-tRNA from HIV-I- and HIV-2-infected cells, in addition lacked the highly modified Wye base on the 3' side of its anticodon. Interestingly, one or both of these tRNAs occur at or near the ribosomal frameshift site in expression of the gag-pol poly protein of each retrovirus. The possible role of the hypomodified base in ribosomal frameshifting is presently being investigated.