MECHANISMS OF NEUROENDOCRINE REGULATION

调查的重点是金融机构的性质和监管 促肾上腺皮质激素释放因子受体及其作用机制 促肾上腺皮质激素释放激素与其他促肾上腺皮质激素分泌调节因子之间的相互作用。 A.CRF受体特性：凝胶电泳法分析 1251-Tyr-OCRF交联的洗涤剂增溶CRF受体 表明该受体是一个带有分子的单一蛋白质 重量为67 kDa。CRF受体的特征是 在不同的靶组织中相似，包括前部和 垂体和皮质的中间叶，杏仁核和 大脑的嗅球。 B.CRF受体调节：先前的研究表明 脑垂体促肾上腺皮质激素释放激素受体下调 肾上腺切除术后血浆ACTH的升高依赖于 下丘脑因子，如CRF和VP。应激期间的研究 慢性前列腺炎后血浆促肾上腺皮质激素一过性升高 动弹不得。血浆促肾上腺皮质激素水平的随后下降 持续的应激伴随着CRF受体的下降- 脑垂体的调节和脱敏。然而， 体内垂体反应性及其增强作用 维甲酸在体外对CRF的作用 监管机构之间相互作用的重要性 ACTH分泌的生理调控。促肾上腺皮质激素释放激素受体 慢性阻塞性肺病患者的中间脑垂体区和脑区的变化 制动应激。 C.促肾上腺皮质激素调节因子的作用机制：以前的研究 Vp对CRF作用的协同作用 包括增强CRF刺激的cAMP产生，提示 蛋白激酶C的激活是其作用机制的一部分 副总统的。对分离的垂体细胞的研究表明，抑制作用 内源性蛋白激酶C可消除VP在大鼠脑缺血再灌注中的作用 促肾上腺皮质激素。此外，VP还能刺激肌醇 磷酸盐的形成和诱导蛋白激酶转位 C从细胞质到细胞膜隔室。

Investigation has focused on the properties and regulation of the corticotropin releasing factor receptors and the mechanism of interaction between CRF and other regulators of ACTH secretion. A. CRF receptor properties: Gel electrophoresis analysis of detergent-solubilized CRF receptors crosslinked with 1251-Tyr-oCRF indicated that the receptor is a single protein with a molecular weight of 67 kDa. The characteristics of the CRF receptor are similar in the different target tissues, including anterior and intermediate lobes of the pituitary and the cortex, amygdala and olfactory bulb of the brain. B. CRF receptor regulation: Previous studies have shown that pituitary CRF receptor down regulation that accompanies the increase in plasma ACTH following adrenalectomy is dependent on hypothalamic factors, such as CRF and VP. Studies during stress showed transient increases in plasma ACTH following chronic immobilization. The subsequent decrease in plasma ACTH in the continuous presence of stress is accompanied by CRF receptor down- regulation and desensitization of the pituitary. However, pituitary responsiveness in vivo as well as the potentiating effect of VP on CRF action in vitro are maintained emphasizing the importance of the interaction between regulators during physiological control of ACTH secretion. CRF receptors in the intermediate pituitary and brain are unchanged during chronic immobilization stress. C. Mechanism of action of ACTH regulators: Previous studies demonstrated that the synergistic effect of VP on CRF action involves potentiation of CRF-stimulated cAMP production suggesting that protein kinase C activation is part of the mechanism of action of VP. Studies in isolated pituitary cells showed that inhibition of endogenous protein kinase C abolishes the effects of VP in the corticotroph. In addition VP was shown to stimulate inositol phosphate formation and to induce translocation of protein kinase C from cytosol to the membrane compartment.