ROLE OF VESICULAR PH IN STORAGE OF NE IN ADRENERGIC SYNAPTIC VESICLES

囊泡 PH 在肾上腺素能突触囊泡 NE 储存中的作用

• 批准号: 3920136
• 负责人: D F BOGDANSKI
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3920136
• 关键词: acidity /alkalinity; adenosine triphosphate; adenosinetriphosphatase; adrenergic receptor; magnesium; membrane potentials; nerve endings; norepinephrine; protein transport; reserpine; synapses; vesicle /vacuole

This laboratory has studied the storage of norepinephrine (NE) in synaptic vesicles in the axoplasm within adrenergic nerve terminals (in situ). Storage depended upon energy utilizing reactions involving ATP at the membranes of vesicles (Bogdanski and Blaszkowski, 1973). The results of other studies have shown that vesicles in situ respond to known inhibitors of the uptake dependent upon Mg++ -ATPase activity in the membranes of isolated vesicles (Bogdanski, 1982; 1983; 1986; 1988). In isolated vesicles, the activity of MS++ -ATPase drives protein transport to generate electrochemical gradients of protons across the vesicle membrane. These gradients drive the transport of NE. Biochemical reagents which block or dissipate the gradients of H+ block uptake and, generally, release NE from vesicles in situ. The past years' work has dealt primarily with the inhibitory effect of ammonia on storage by vesicles in situ. This alkalinizing reagent for vesicles blocks ATP-dependent uptake, and releases NE from vesicles. A series of such responses were compared with those of reserpine, which blocks uptake and releases NE but has no effect on electrochemical gradients of H+.

该实验室研究了去甲肾上腺素（NE）在 肾上腺素能神经末梢内轴浆中的突触小泡 (in situ）。 存储依赖于能量利用反应 涉及囊泡膜上的ATP（Bogdanski和 Blaszkowski，1973）。 其他研究的结果表明， 囊泡在原位响应于已知的摄取抑制剂 Mg~（++）-ATP酶活性的依赖性 囊泡（Bogdanski，1982; 1983; 1986; 1988）。 离体 在囊泡中，MS ++-ATP酶的活性驱动蛋白质转运到 产生质子穿过囊泡的电化学梯度 膜的 这些梯度驱动NE的运输。 生化 阻断或消散H+梯度的试剂阻断吸收 并且通常从囊泡原位释放NE。 过去几年的工作主要涉及抑制效应 氨在囊泡原位储存。 这种碱化 囊泡试剂阻断ATP依赖性摄取并释放NE 从囊泡。 一系列这样的反应与那些 利血平，它阻止吸收和释放NE，但没有影响 H+的电化学梯度。