APPLICATIONS OF A MOLECULAR BIOLOGY CORE FACILITY

肽/蛋白质测序仪、DNA测序仪和DNA 所需的合成器将用于建立DNA和蛋白质 分析医学院的核心设施。这个 由这些最先进的设备提供的实验功能 仪器将促进美国国立卫生研究院的持续生产力 获得资助的项目涉及：(1)对人类基因组的遗传分析 专性胞内寄生普氏立克次体； 人癌胚抗原编码基因的鉴定 哺乳动物胎儿和肿瘤细胞及其与 原癌基因；(3)兔胎盘松弛素的特性； (4)鼠伤寒沙门氏菌基因调控特性 刺激；(5)角膜单纯疱疹病毒的致病机制 (6)血管内皮细胞p140的序列分析 膜；(7)独特型--的分离和特性 特异性辅助因子基因；(8)环状病毒的生物学作用 核苷酸磷酸二酯酶系统；(9)作用机制 环境化学品的β细胞毒性；(10) 普氏立克次体ADP/ATP的特性研究 转位酶；(11)DNA的形成和生物学后果 加合物。 在NIH资金悬而未决的情况下，更多的项目将受益 从这一核心设施的存在谈起：(12) 配子发生过程中的分子和遗传机制 在小鼠中；(13)Galago Sine的表达和反向定位 元素；(14)磷脂转运蛋白在肺中的作用； (15)肺细胞组织发生的过程；(16)特征 哺乳动物发育过程中的染色体外DNA 老化；(17)提纯、重组和分子/化学 线粒体三羧酸转运蛋白的特性； (18)甲基化DNA病毒的转录调控。

The peptide/protein sequencer, DNA sequencer, and DNA synthesizer requested will be used to establish a DNA and protein analysis core facility in the college of Medicine. The experimental capabilities provided by these state-of-the-art instruments will facilitate the continued productivity of NIH funded projects dealing with the: (1) genetic analysis of the obligate intracellular parasite Rickettsia prowazekii; (2) identification of the oncofetal antigen encoding gene in mammalian fetal and tumor cells and comparison with protooncogenes; (3) characterization of rabbit placental relaxin; (4) characterization of Salmonella typhimurium genetic regulatory stimulons; (5) pathogenesis of corneal Herpes simplex virus infection; (6) sequence analysis of p140 from smooth muscle membranes; (7) isolation and characterization of an idiotype- specific helper factor gene; (8) biological role of the cyclic nucleotide phosphodiesterase enzyme system; (9) mechanisms of beta cell toxicity by environmental chemicals; (10) characterization of the Rickettsia prowazekii ADP/ATP translocase; (11) formation and biological consequences of DNA adducts. Additional projects, with NIH funding pending, that will benefit from the presence of this core facility deal with the: (12) molecular and genetic mechanisms involved during gametogenesis in the mouse; (13) expression and retroposition of Galago SINE elements; (14) role of phospholipid transfer protein in the lung; (15) process of lung cell histogenesis; (16) characterization of extrachromosomal DNAs during mammalian development and aging; (17) purification, reconstitution, and molecular/chemical characterization of the mitochondrial tricarboxylate transporter; (18) transcriptional regulation of a methylated DNA virus.