TRANSMISSION ELECTRON MICROSCOPE

我们将调查涉及多个DNA的DNA交易- 蛋白质相互作用和复杂的DNA拓扑结构。直接式 由电子显微镜提供的可视化是一种 选择研究这些交易是因为DNA折叠， 可以跟随缠绕或解离和DNA拓扑 直接检查。因此，我们要求一个新的 透射式电子显微镜将提供特殊的 功能和极大增强的可用性。研究课题 包括：通过噬菌体lambda和Tn3进行定点重组 噬菌体Lambda、P4和BPV复制DNA 和SV40病毒；增强子介导的转录启动 BPV。这些过程涉及多种dna-蛋白质关联。 以及长距离的DNA相互作用。我们的分析应该会导致一个 在我们对这些特定系统的理解方面取得了实质性进展 以及这种复杂的DNA交易的一般性质。

We will investigate DNA transactions that involve multiple DNA- protein interactions and complex DNA topology. The direct visualization provided by electron microscopy is the method of choice for the study of these transactions because DNA folding, winding, or unwinding can be followed and DNA topology examined directly. Therefore, we are requesting a new transmission electron microscope that will provide special features and vastly enhanced availability. Topics for study include: site-specific recombination by phage lambda and Tn3 resolvase; DNA replication by phages lambda and P4 and by BPV and SV40 viruses; enhancer-mediated initiation of transcription by BPV. These processes involve multiple DNA-protein associations and long range DNA interactions. Our analysis should lead to a substantial advance in our understanding of those specific systems and the general nature of such complex DNA transactions.