TRANSMISSION ELECTRON MICROSCOPE

我们将调查涉及多个DNA的DNA交易- 蛋白质相互作用和复杂的DNA拓扑结构。 直接 电子显微镜提供的可视化是 选择这些交易的研究，因为DNA折叠， 缠绕，或解绕可以遵循和DNA拓扑 直接检查。 因此，我们要求新的 透射电子显微镜，将提供特殊的 功能和大大增强的可用性。 研究课题 包括：噬菌体λ和Tn 3位点特异性重组 解旋酶;通过λ和P4以及通过BPV的DNA复制 和SV 40病毒;增强子介导的转录起始， BPV。 这些过程涉及多个DNA-蛋白质缔合 和长距离DNA相互作用。 我们的分析应该会得出 我们对这些特定系统的理解取得了实质性进展 以及这种复杂的DNA交易的一般性质。

We will investigate DNA transactions that involve multiple DNA- protein interactions and complex DNA topology. The direct visualization provided by electron microscopy is the method of choice for the study of these transactions because DNA folding, winding, or unwinding can be followed and DNA topology examined directly. Therefore, we are requesting a new transmission electron microscope that will provide special features and vastly enhanced availability. Topics for study include: site-specific recombination by phage lambda and Tn3 resolvase; DNA replication by phages lambda and P4 and by BPV and SV40 viruses; enhancer-mediated initiation of transcription by BPV. These processes involve multiple DNA-protein associations and long range DNA interactions. Our analysis should lead to a substantial advance in our understanding of those specific systems and the general nature of such complex DNA transactions.