STUDIES ON THE ACTIVE SITES OF CYTOCHROMES P-450

细胞色素 P-450 活性位点的研究

• 批准号: 3942797
• 负责人: K KORZEKWA
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942797
• 关键词: chemical reaction; cytochrome P450; enzyme structure; enzyme substrate; hydroxylation; isozymes; stereochemistry; steroid analog; steroid metabolism

Cytochrome P-450a is a P-450 isozyme showing a high degree of regiospecificity of the 7 alpha-hydroxylation of steroids. The steric and electronic binding properties responsible for this specificity will be probed by evaluating the metabolism of several steroid analogs or fragments. Models used in this study include aliphatic and unsaturated ring systems steroid fragments, and a potential heme-binding optically active steroid analog. The regioselectivity, chiral and prochiral selectivity, and kinetics of the metabolism of these substrates will be used to evaluate the geometric properties of the P-450a active site.

细胞色素P-450a是一种P-450同工酶