PERTUSSIS HEAT LABILE TOXIN (HLT)--ISOLATION AND CHARACTERIZATION

百日咳杆菌产生几种蛋白质，除了 百日咳毒素，具有药理活性(毒素)。其中之一 这些以前被称为皮肤病毒素，现在 被命名为耐热性毒素(HLT)，已被高度分离 纯净的形式。它的结构和生物活性正在 特色化的。HLT由单一的多肽链组成， 分子量约150,000 D实验室致死剂量 老鼠的体重异常低，范围约为10pg，使其成为 所研究的大多数有毒蛋白质。单抗已经被 对小鼠脑内攻击具有免疫力的分离株 百日咳杆菌提供证据表明HLT可能是一种 额外的保护性抗原。 注射亚致死剂量的HLT引起异常病理改变 变化包括红骨髓的迅速耗尽。B.百日咳 相应地，只产生微量的HLT，DNA 重组技术必须应用于这一问题 为了考虑将该毒素用于临床而生产 评估。

Bordetella pertussis produces several proteins, in addition to pertussis toxin, with pharmacological activities (toxins). One of these, previously called the dermonecrotic toxin and now designated as heat labilt toxin (HLT), has been isolated in highly purified form. Its structural and biological activities are being characterized. HLT is composed of a single polypeptide chain, molecular weight ca. 150,000 D. the lethal dose in laboratory mice is unusually low, ranging about 10 pg, making it one of the most toxic proteins studied. Monoclonal antibodies have been isolated which confer immunity to intracerebral challenge of mice with B. pertussis providing evidence that HLT might serve as an additional protective antigen. Injection of sublethal doses of HLT induces unusual pathological changes including rapid depletion of the red marrow. B. pertussis produces only trace amounts of HLT, accordingly, DNA recombinant technology must be applied to this problem of production on order to consider the use of this toxin for clinical evaluation.