PERTUSSIS HEAT LABILE TOXIN (HLT)--ISOLATION AND CHARACTERIZATION

百日咳热不稳定毒素 (HLT)——分离和表征

• 批准号: 3942107
• 负责人: R D SEKURA
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942107
• 关键词: Bordetella pertussis; antibacterial antibody; bacterial toxins; bactericidal immunity; bioassay; high performance liquid chromatography; immunological substance; monoclonal antibody; passive immunization; pertussis; thermostability; virulence

Bordetella pertussis produces several proteins, in addition to pertussis toxin, with pharmacological activities (toxins). One of these, previously called the dermonecrotic toxin and now designated as heat labilt toxin (HLT), has been isolated in highly purified form. Its structural and biological activities are being characterized. HLT is composed of a single polypeptide chain, molecular weight ca. 150,000 D. the lethal dose in laboratory mice is unusually low, ranging about 10 pg, making it one of the most toxic proteins studied. Monoclonal antibodies have been isolated which confer immunity to intracerebral challenge of mice with B. pertussis providing evidence that HLT might serve as an additional protective antigen. Injection of sublethal doses of HLT induces unusual pathological changes including rapid depletion of the red marrow. B. pertussis produces only trace amounts of HLT, accordingly, DNA recombinant technology must be applied to this problem of production on order to consider the use of this toxin for clinical evaluation.

百日咳博德特氏菌产生几种蛋白质，除了 具有药理活性的百日咳毒素（毒素）。 之一 这些，以前称为皮肤坏死毒素，现在 被命名为热不稳定毒素（HLT），已被高度分离， 纯化形式。 它的结构和生物活性正在被 表征了 HLT由单一多肽链组成， 分子量ca. 15万第纳尔实验室致死剂量 小鼠的血糖异常低，范围约为10 pg，使其成为 研究的大多数有毒蛋白质。 单克隆抗体已经 其赋予对小鼠脑内攻击的免疫力 和B在一起百日咳提供的证据表明，HLT可能作为一种 额外的保护性抗原。 注射亚致死剂量的HLT诱导异常病理学变化， 包括红骨髓快速耗竭的变化。 B。百日咳 仅产生微量的HLT，因此，DNA 重组技术必须应用于这个问题， 为了考虑将这种毒素用于临床， 评价