KINETICS, REGULATION AND MECHANISMS OF BIOCHEMICAL REACTIONS

(1)测试直接迁移机制的实验 对糖酵解途径中的代谢物的研究表明 用于支持拟议机制的数据值得怀疑。 与醛缩酶与α-形成络合物的报道相反- 甘油-3-磷酸脱氢酶和促进 脱氢酶直接转移催化反应 醛缩酶结合的底物，二羟丙酮磷酸，我们发现 这种醛缩酶通过以下途径抑制脱氢酶催化的反应 α-甘油-3-磷酸底物的脱除 脱氢酶。此外，瞬时动力学数据表明， 报告了来自阿尔法的NADH的一次有序传输速率 甘油-3-磷酸脱氢酶为乳酸脱氢酶 一种由两个反应组成的复合体，其中一个是由于NADH与 乳酸脱氢酶和另一种由于络合物形成 在两种脱氢酶之间。 (2)Ca(II)与若干二价金属的结合常数 测定了一类钙(II)指示剂的离子 旨在探测细胞内钙离子浓度。 (3)已经发展了一种理论来解释 膜电位波动对膜电位的影响 膜结合蛋白。这一理论提供了一个合理的 能量转导机制。此外，一个振荡的 电脉冲装置正在建设中，以实现 通过实验验证了该理论的正确性。 (4)异肽酶的纯化和鉴定工作正在进行中。

(1) Experiments designed to test the direct transfer mechanism for metabolites in the glycolytic pathway have revealed that the data used to support the proposed mechanism are questionable. Contrary to the report that aldolase forms a complex with alpha- glycerol-3-phosphate dehydrogenase and facilitates the dehydrogenase catalyzed reaction through direct transfer of aldolase-bound substrate, dihydroxyacetone phosphate, we found that aldolase inhibits the dehydrogenase-catalyzed reaction by removing substrate for alpha-glycerol-3-phosphate dehydrogenase. In addition, transient kinetic data show that the reported first-ordered transfer rate of NADH from alpha- glycerol-3-phosphate dehydrogenase to lactate dehydrogenase is a composite of two reactions, one due to free NADH binding to lactate dehydrogenase and the other due to complex formation between the two dehydrogenases. (2) Binding constants for Ca(II) and a number of divalent metal ions have been determined for a family of Ca(II) indicators designed to probe intracellular calcium concentration. (3) A theory has been developed to account for the effect of oscillating of fluctuating membrane potential on the function of membrane-bound proteins. The theory provides a reasonable mechanism for energy transduction. In addition, an oscillating electric pulse apparatus is being constructed to carry out experiments to test the validity of the theory. (4) Work is in progress to purify and characterize isopeptidase.