KINETICS, REGULATION AND MECHANISMS OF BIOCHEMICAL REACTIONS

(1)设计用于测试直接转移机制的实验 对糖酵解途径中代谢物的研究表明， 用于支持拟议机制的数据值得怀疑。 与报道的醛缩酶与α形成复合物相反， 甘油-3-磷酸脱氢酶和促进 脱氢酶催化反应通过直接转移 醛缩酶结合的底物，磷酸二羟丙酮，我们发现 醛缩酶抑制了醛缩酶催化的反应， 除去α-甘油-3-磷酸的底物 脱氢酶。 此外，瞬态动力学数据表明， 报道的NADH从α- 甘油-3-磷酸脱氢酶转化为乳酸脱氢酶， 两个反应的复合物，一个是由于游离的NADH结合到 乳酸脱氢酶和其他由于复杂的形成 在两个十字架之间。 (2)Ca（II）和一些二价金属的结合常数 离子已被确定为一个家庭的钙（II）指示剂 用来探测细胞内钙浓度 (3)已经发展出一种理论来解释 波动膜电位的振荡 膜结合蛋白。 该理论提供了一个合理的 能量转换机制。 此外，振荡 电脉冲装置正在建造， 实验来检验理论的有效性。 (4)异肽酶的纯化和表征工作正在进行中。

(1) Experiments designed to test the direct transfer mechanism for metabolites in the glycolytic pathway have revealed that the data used to support the proposed mechanism are questionable. Contrary to the report that aldolase forms a complex with alpha- glycerol-3-phosphate dehydrogenase and facilitates the dehydrogenase catalyzed reaction through direct transfer of aldolase-bound substrate, dihydroxyacetone phosphate, we found that aldolase inhibits the dehydrogenase-catalyzed reaction by removing substrate for alpha-glycerol-3-phosphate dehydrogenase. In addition, transient kinetic data show that the reported first-ordered transfer rate of NADH from alpha- glycerol-3-phosphate dehydrogenase to lactate dehydrogenase is a composite of two reactions, one due to free NADH binding to lactate dehydrogenase and the other due to complex formation between the two dehydrogenases. (2) Binding constants for Ca(II) and a number of divalent metal ions have been determined for a family of Ca(II) indicators designed to probe intracellular calcium concentration. (3) A theory has been developed to account for the effect of oscillating of fluctuating membrane potential on the function of membrane-bound proteins. The theory provides a reasonable mechanism for energy transduction. In addition, an oscillating electric pulse apparatus is being constructed to carry out experiments to test the validity of the theory. (4) Work is in progress to purify and characterize isopeptidase.