MUTATIONS IN CONTROL REGIONS THAT REGULATE SV40 TRANSCRIPTION

SV40的400个碱基对区域包含早期和 晚期转录和DNA复制的起源。我们已经确定了一个 位于5‘端起始点上游30个碱基对的替代塔塔盒 在体外和体内发挥调节作用的晚期转录 后期转录水平。早期转录水平受到影响 富含GC的序列及其与塔塔盒子的距离 位于早期5‘起点的上游。

A 400 base pair region in SV40 contains the promoter elements of early and late transcription and the origin of DNA replication. We have identified a surrogate TATA box that lies 30 base pairs upstream of the 5' start site of late transcription that functions in vitro and in vivo to regulate the level of late transcription. The level of early transcription is effected by GC rich sequences and their distance from the TATA box that lies upstream of the early 5' start site.