DIFFERENTIATION OF HUMAN RETINOBLASTOMA

Y-79人视网膜母细胞瘤细胞株的单层培养 最近，通过在特定覆盖的基质上培养细胞 多聚-0-赖氨酸和纤维连接蛋白。对这样附着的细胞进行了研究 光镜和电子显微镜、免疫荧光和免疫过氧化物酶 与各种药物分化前后，如 二丁酰环AMP(DBC-AMP)和丁酸钠(NaBUT)。 采用有血清培养和无血清培养。仅仅是依附于 细胞导致了轻微的形态分化，这是 在添加了代理后得到了增强。带进程的圆形单元格，如 同时出现扁平的、贴壁的细胞。特定的神经元和 在不同条件下，细胞均表达神经胶质标记物。此外， 神经元、光感受器、神经胶质细胞和 可观察到着色细胞。结果支持区分 原始视网膜母细胞瘤细胞，如Y-79系的细胞，转化为 正常人视网膜的各种细胞类型，即光感受器，神经元， Muller和着色的上皮细胞。这些发现不仅支持 视网膜母细胞瘤起源于视盘原始细胞，但也 提供一个系统来研究细胞相互作用和肿瘤细胞对 各种治疗剂。

Monolayer cultures of the Y-79 human retinoblastoma cell line were obtained recently by growing the cells on substrata specifically covered with poly-0-lysine and fibronectin. The cells thus attached were studied by light and electron microscopy, immunofluorescence and immunoperoxidase before and after differentiation with various agents, such as dibutyryl-cyclic AMP (dbc-AMP) and sodium butyrate (Nabut). Serum-supplemented and serum-free media were used. Mere attachment of the cells resulted in minor degrees of morphologic differentiation, which was enhanced after the addition of the agents. Round cells with processes, as well as flat, substrate-adherent cells appeared. Specific neuronal and glial markers were expressed by the cells in various conditions. Moreover, ultrastructural characteristics of neuronal, photoreceptor, glial and pigmented cells were observed. The results support differentiation of primitive retinoblastoma cells, such as those of the Y-79 line, into various cell types of normal human retina, i.e. photoreceptor, neuronal, Muller and pigmented epithelial cells. These findings not only support origin of retinoblastoma from a primitive cell of the optic disc, but also offer a system to study cell interactions and tumor cell responses to various therapeutic agents.