ONDCOGENESIS AND INSERTION MEDIATED MUTAGENESIS IN TRANSGENIC MICE

我们的实验室研究基因控制的机制在生活中 哺乳动物有机体。 通过显微注射将特定的基因导入 小鼠的生殖系，并在小鼠的生殖系中测定这些基因的表达。 产生转基因动物。 为此，器官筛查在 从胎儿到成人的不同发育阶段。 两个问题 研究了 第一个涉及转基因动物的肿瘤发生。 一 融合基因，由小鼠α晶体蛋白启动子和 将SV 40肿瘤抗原的编码序列插入小鼠生殖细胞中， 线 在迄今为止产生的所有小鼠品系中均发生了透镜肿瘤。 肿瘤 细胞表达SV 40 T抗原。 细胞转化的开始 与出生中期的透镜分化相一致。 第二 问题涉及在我们的一个菌株中观察到的插入诱变。 转基因小鼠 一个小产仔数的显性表型被证明， 与染色体6/17相互易位相吻合，并且该基因 通过原位杂交将插入物定位到重排的 染色体

Our laboratory investigates mechanisms of gene control in the living mammalian organism. Specific genes are introduced by microinjection into the germ line of mice, and expression of these genes is assayed in the resulting transgenic animals. To this end, organ screens are performed at various stages of development from fetus to adult. Two problems were studied. The first dealt with oncogenesis in the transgenic animal. A fused gene, consisting of the mouse Alpha crystallin promoter and the coding sequences of the SV40 tumor antigens, was inserted in the mouse germ line. Lens tumors developed in all mouse lines generated so far. Tumor cells expressed the SV40 T antigen. The onset of cell transformation coincided with lens differentiation during mid-getation. The second problem concerns insertional mutagenesis observed in one of our strains of transgenic mice. A dominant phenotype of small litter size was shown to coincide with a chromosome 6/17 reciprocal translocation, and the gene insert was localized by in situ hybridization to one of the rearranged chromosomes.