INTRUMENTATION DEVELOPMENT FOR FLUORESCENCE SPECTROSCOPY

介绍了一种时间分辨荧光光谱仪的自动控制系统。 开发的目的是实现荧光光谱的快速收集。几个 基于激光的系统的外围设备由惠普控制 1000系列小型机，包括采集、传输和存储 数据的选择、样本的选择和自动定位 用于波长选择的单色仪。对这些外围设备的控制 允许以比手动方式更快的速度收集数据 系统，操作员几乎不干预或不干预，并且还允许最大 在设计和增强实验方面的灵活性。 结合计算机控制和采集的特定实验 包括在采样和参考灯数据采集之间交替 固定波长实现荧光衰减曲线，并采集 快速扫描时来自样品的时间分辨荧光数据 通过一系列波长。由于使用了模块化编程，因此新的 可以很容易地配置实验。

Automatic control of a time-resolved fluorescence spectrophotometer was developed to enable the rapid collection of fluorescence spectra. Several peripherals of the laser based system are controlled by a Hewlett-Packard Series 1000 mini-computer, including the acquisition, transfer, and storage of data, the selection of samples, and the automatic positioning of monochromators for wavelength selection. The control of these peripherals allows data to be collected more quickly than is possible with a manual system, with little or no operator intervention, and also allows maximum flexibility in the design and enhancement of experiments. Specific experiments that incorporate computer control and acquisition include alternating between sample and reference lamp data acquisition at fixed wavelengths to achieve fluorescence decay curves, and collecting time-resolved fluorescence data from a sample while rapidly scanning through a series of wavelengths. Because modular programming was used, new experiments can be easily configured.